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Casp6 (NM_009811) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203708
Casp6 (Myc-DDK-tagged) - Mouse caspase 6 (Casp6)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Casp6
Synonyms CASP-6; mCAS; Mch2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203708 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAGAAACCGATGGCTTCTACAAAAGTAGGGAAGTGTTCGATCCAGCCGAGCAGTACAAGATGGACC
ACAAGAGGAGAGGAGTTGCCCTCATCTTCAATCACGAGAGGTTCTTTTGGCACCTGACCCTCCCAGAGAG
GCGGGGCACCAACGCAGACAGAGACAACCTGACTCGCAGGTTTTCAGATCTAGGATTTGAAGTGAAATGC
TTTAACGACCTCAGAGCAGAAGAACTCCTGCTCAAAATTCACGAGGTGTCGACTTCAAGCCACATAGATG
CCGATTGCTTCATCTGTGTCTTCCTGAGCCACGGGGAAGGCAACCACGTTTACGCATACGACGCCAAAAT
TGAAATTCAGACGTTGACTGGCTTGTTCAAAGGAGACAAATGTCAGAGCCTGGTTGGAAAACCCAAGATA
TTTATCATCCAGGCCTGTCGGGGCAGCCAGCATGACGTACCTGTGGTTCCCCTGGACGTGGTGGATCACC
AGACAGACAAGCTGGACAACGTGACCCAGGTGGATGCTGCGTCTGTGTACACGCTGCCGGCAGGGGCAGA
CTTCCTCATGTGCTACTCTGTCGCAGAAGGGTATTACTCTCACCGAGAAACTGTGAATGGCTCCTGGTAC
ATTCAGGATTTGTGTGAGATGCTGGCGAGGTACGGCAGTTCCCTGGAGTTCACGGAGCTGCTCACGCTGG
TGAACAGAAAGGTCTCTCAGCGCCGCGTGGACTTCTGCAAAGACCCGGATGCAATCGGCAAGAAGCAGGT
GCCCTGCTTCGCCTCAATGCTGACCAAAAAGCTGCATTTCTGTCCCAAACCTAGCAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203708 protein sequence
Red=Cloning site Green=Tags(s)

MTETDGFYKSREVFDPAEQYKMDHKRRGVALIFNHERFFWHLTLPERRGTNADRDNLTRRFSDLGFEVKC
FNDLRAEELLLKIHEVSTSSHIDADCFICVFLSHGEGNHVYAYDAKIEIQTLTGLFKGDKCQSLVGKPKI
FIIQACRGSQHDVPVVPLDVVDHQTDKLDNVTQVDAASVYTLPAGADFLMCYSVAEGYYSHRETVNGSWY
IQDLCEMLARYGSSLEFTELLTLVNRKVSQRRVDFCKDPDAIGKKQVPCFASMLTKKLHFCPKPSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009811
ORF Size 828 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009811.2, NP_033941.2
RefSeq Size 1455 bp
RefSeq ORF 831 bp
Locus ID 12368
UniProt ID O08738
Cytogenetics 3 G3
MW 31.6 kDa
Summary This gene encodes a member of the cysteine proteases that plays important roles in regulating apoptosis and neurodegeneration. The encoded protein is involved in the transmission of pain and axonal degeneration. Genetic deletion of this gene in mice results in the delay of axon pruning and protects from axon degeneration. provided by RefSeq, Apr 2015
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Documents
Resources
"NM_009811" in other vectors (4)
SKU Description Size Price
MC205605 Casp6 (untagged) - Mouse caspase 6 (Casp6), (10ug) 10 ug
$300.00
MG203708 Casp6 (tGFP-tagged) - Mouse caspase 6 (Casp6) 10 ug
$489.00 $500.00
MR203708L3 Lenti ORF clone of Casp6 (Myc-DDK-tagged) - Mouse caspase 6 (Casp6) 10 ug
$600.00
MR203708L4 Lenti ORF clone of Casp6 (mGFP-tagged) - Mouse caspase 6 (Casp6) 10 ug
$600.00

Plasmid Map for MR203708

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203708
Circular map for MR203708

file-alt Citations

Nitrite protects neurons against hypoxic damage through <i>S</i>-nitrosylation of caspase-6
Chen, Liu, Liu et al
Antioxid. Redox Signal. (2018)
AI Summary: In this study, the OriGene caspase-6 plasmid (MR203708) was used to investigate the role of caspase-6 in hypoxia-induced neuronal injury and the protective effects of nitrite.
Snippet: The small interfering RNA (siRNA) EGFP, caspase‐6 (L‐063186‐00‐0005) or caspase‐3 (L‐ 043042‐00‐0005) were all purchased from Dharmacon. siRNA (100 nM) was transfected into N2a cells using a Lonza Amaxa Nucleofector Kit (VCA‐1003) following manufacturer’s directions. The caspase‐6 plasmid (MR203708) was purchased from OriGene
Showing 1-1 of 1 papers.
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