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Brix1 (NM_026396) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203702
Brix1 (Myc-DDK-tagged) - Mouse BRX1, biogenesis of ribosomes, homolog (S. cerevisiae) (Brix1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Brix1 or NM_026396"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Brix1
Synonyms 1110064N10Rik; Bxdc2; C76935
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203702 representing NM_026396
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGACCAAGAGAAAGCGGCGTGGAGGCTTGGAGGTTCAAGCGAAGAAGCCAAAAAGGAGCTCGA
AAGATGCCGGGCAGCCAGCTAAGCAGGCCGATGTGGCCAAAGAGGCGGAGGAGGAAAATAGAGATCGTAT
TCCAGGTCCGGTTTGCAAGGGCAAATGGAAAAATAAGGAACGGATCCTCATCTTTTCTTCCAGAGGAATA
AATTTCAGAACAAGACACTTAATGCAGGACTTGAGAATGTTGATGCCTCATTCTAAAGCAGATACTAAAA
TGGATCGTAAAGATAAATTATTTGTGATAAATGAGGTCTGTGAAATGAAAAATTGCAACAAATGCATCTA
CTTTGAAGCTAAGAAAAAACAGGATCTCTATATGTGGCTTTCAAATTCACCTCATGGTCCATCTGCCAAA
TTCTTGGTTCAAAATATTCATACCCTGGCTGAATTAAAGATGACTGGAAACTGTTTGAAAGGTTCTCGGC
CCCTTTTGTCTTTTGACCCTGCTTTTGATGACTTGCCACATTATGCTTTGTTAAAGGAATTCTTAATTCA
GATCTTCAGTACGCCACGATACCATCCCAAGAGTCAGCCGTTTGTGGACCACGTGTTTACGTTCACCATT
CTGGATAACAGGATATGGTTCCGGAACTTCCAGATTATAGAAGAAGACGCTGCTCTAGTGGAAATAGGAC
CTCGTTTTGTTTTAAATCTCATAAAGATTTTTCAGGGAAGCTTTGGAGGACCAACCTTATATGAAAATCC
TCATTACCAGTCTCCAAACATGCATCGGCGTGTCATAAGATCCATCACAGCTGCCAAGTATAGAGAGAGA
CAGCAAGTCAAAGATGTTCAGAAACTGAGGAAGAAAGAACCAAAGACTATCCTTCCACATGACCCCACTG
CAGACGTGTTTGTTATACCAGCGGAGGAGAAGCCAGTAGAAATACAGTGGGTGAAACCAGAGCCTAAAGT
AGATCTGAAAGCAAGGAAGAGACGGATTTACAAAAGGCATCGGAAACTGCAACAGAAGATGAGCAGAGGG
AGTGCAAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026396
ORF Size 1059 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026396.3, NP_080672.3
RefSeq Size 2654 bp
RefSeq ORF 1062 bp
Locus ID 67832
UniProt ID Q9DCA5
Cytogenetics 15 A1
MW 41.7 kDa
Summary Required for biogenesis of the 60S ribosomal subunit.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026396" in other vectors (4)
SKU Description Size Price
MC210632 Brix1 (untagged) - Mouse BRX1, biogenesis of ribosomes, homolog (S. cerevisiae) (Brix1), (10ug) 10 ug
$503.00
MG203702 Brix1 (tGFP-tagged) - Mouse brix domain containing 2 (Bxdc2) 10 ug
$657.00
MR203702L3 Lenti ORF clone of Brix1 (Myc-DDK-tagged) - Mouse BRX1, biogenesis of ribosomes, homolog (S. cerevisiae) (Brix1) 10 ug
$757.00
MR203702L4 Lenti ORF clone of Brix1 (mGFP-tagged) - Mouse BRX1, biogenesis of ribosomes, homolog (S. cerevisiae) (Brix1) 10 ug
$757.00

Plasmid Map for MR203702

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203702
Circular map for MR203702

file-alt Citations

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