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Pef1 (NM_026441) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203692
Pef1 (Myc-DDK-tagged) - Mouse penta-EF hand domain containing 1 (Pef1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pef1
Synonyms 2600002E23Rik; Peflin
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203692 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGCTATCCAAACGGACAGAGTTGCCCAGGAGCTGCAGGACAGGTGCCTGGAGTACCCCCGGGGG
GCTATTATCCTGGTCCTCCCCATGGTGGGGGCCAGTATGGCAGTGGACTGCCCCCAGGTGGTGGCTATGG
AGCTCCTGCCCCTGGAGGACCTTATGGATACCCCAGTGCTGGAGGAGTCCCCTCGGGAACTCCAAGTGGA
CCATATGGCGGTATACCTCCAGGGGGTCCCTATGGTCAGCTACCTCCAGGGGGTCCCTACGGTACCCAGC
CTGGACATTATGGACAGGGTGGTGTCCCCCCGAATGTGGATCCTGAGGCCTACTCCTGGTTCCAGTCAGT
GGATGCCGATCACAGTGGCTATATCTCCCTCAAGGAGCTGAAGCAGGCCCTAGTCAACTCCAACTGGTCC
TCATTCAATGACGAGACATGCCACATGATGATAAACATGTTTGACAAGACCAAGTCTGGCCGCATTGATG
TCGCCGGCTTCTCAGCCTTATGGAAATTCCTCCAGCAGTGGAGGAACCTCTTTCAGCAGTATGACCGGGA
CCGCTCGGGCTCCATTAGCTCCACAGAGCTGCAGCAAGCGCTCTCCCAGATGGGCTACAACCTGAGCCCT
CAGTTCACGCAGCTCCTGGTTTCCCGGTACTGCGCACGCTCTGCTATTCCCGCCATGCAGCTTGACTGCT
TCATCAAGGTGTGTACCCAGCTGCAGGTGTTGACTGAGGCCTTCCGGGAAAAGGATACCGCTGTACAGGG
CAACATCCGGCTCAGCTTTGAGGACTTTGTCACCATGACGGCTTCAAGGATGCTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203692 protein sequence
Red=Cloning site Green=Tags(s)

MASYPNGQSCPGAAGQVPGVPPGGYYPGPPHGGGQYGSGLPPGGGYGAPAPGGPYGYPSAGGVPSGTPSG
PYGGIPPGGPYGQLPPGGPYGTQPGHYGQGGVPPNVDPEAYSWFQSVDADHSGYISLKELKQALVNSNWS
SFNDETCHMMINMFDKTKSGRIDVAGFSALWKFLQQWRNLFQQYDRDRSGSISSTELQQALSQMGYNLSP
QFTQLLVSRYCARSAIPAMQLDCFIKVCTQLQVLTEAFREKDTAVQGNIRLSFEDFVTMTASRML

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026441
ORF Size 825 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026441.2, NM_026441.3, NM_026441.4, NP_080717.2
RefSeq Size 1551 bp
RefSeq ORF 828 bp
Locus ID 67898
UniProt ID Q8BFY6
Cytogenetics 4 D2.2
MW 29.3 kDa
Summary Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats. In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification. Its role in the heterodimer formed with PDCD6 is however unclear: some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium. Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 (By similarity). Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 (By similarity).UniProtKB/Swiss-Prot Function
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"NM_026441" in other vectors (4)
SKU Description Size Price
MC202098 Pef1 (untagged) - Mouse penta-EF hand domain containing 1 (Pef1), (10ug) 10 ug
$300.00
MG203692 Pef1 (tGFP-tagged) - Mouse penta-EF hand domain containing 1 (Pef1) 10 ug
$500.00
MR203692L3 Lenti ORF clone of Pef1 (Myc-DDK-tagged) - Mouse penta-EF hand domain containing 1 (Pef1) 10 ug
$600.00
MR203692L4 Lenti ORF clone of Pef1 (mGFP-tagged) - Mouse penta-EF hand domain containing 1 (Pef1) 10 ug
$600.00

Plasmid Map for MR203692

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR203692
Circular map for MR203692

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