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Uqcrfs1 (NM_025710) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203679
Uqcrfs1 (Myc-DDK-tagged) - Mouse ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 (Uqcrfs1), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Uqcrfs1
Synonyms 4430402G14Rik; AI875505
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203679 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGTCGGTCGCCGCCCGCTCGGGCCCGTTCGCGCCCGTCCTATCGGCCACTTCCCGCGGGGTGGCGG
GCGCGCTGCGTCCTCTGCTGCAAGGCGCGGTGCCCGCCGCCTCGGAGCCACCTGTTCTGGATGTGAAGCG
ACCCTTCCTGTGCCGCGAGTCCCTGAGTGGCCAGGCCGCAGCCCGGCCTTTGGTGGCCACGGTGGGCCTG
AACGTTCCTGCTTCTGTCCGTTTTTCCCATACAGATGTCAAGGTGCCCGACTTCTCTGACTATCGTCGTG
CTGAAGTTCTTGATAGCACAAAATCTTCTAAAGAAAGCAGTGAGGCTAGAAAAGGCTTCTCTTACCTGGT
AACTGCAACTACTACTGTGGGTGTTGCTTATGCGGCCAAAAATGTGGTCTCCCAGTTTGTTTCCAGCATG
AGTGCTTCTGCTGACGTACTGGCCATGTCGAAGATCGAGATCAAGTTGTCTGATATCCCTGAAGGGAAGA
ACATGGCTTTTAAATGGAGAGGCAAACCTCTGTTTGTGCGCCATAGAACCAAGAAGGAGATTGACCAGGA
AGCTGCAGTCGAAGTGTCCCAGTTAAGGGACCCACAGCATGATTTAGATCGAGTAAAGAAACCTGAATGG
GTTATTCTGATAGGTGTCTGTACTCATCTTGGTTGTGTACCCATTGCAAACGCAGGAGATTTTGGTGGCT
ATTATTGCCCCTGCCATGGGTCACACTATGACGCCTCTGGCAGGATCAGGAAGGGCCCTGCACCTCTCAA
CCTGGAAGTGCCTGCCTACGAGTTCACCAGTGATGATGTAGTTGTTGTGGGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203679 protein sequence
Red=Cloning site Green=Tags(s)

MLSVAARSGPFAPVLSATSRGVAGALRPLLQGAVPAASEPPVLDVKRPFLCRESLSGQAAARPLVATVGL
NVPASVRFSHTDVKVPDFSDYRRAEVLDSTKSSKESSEARKGFSYLVTATTTVGVAYAAKNVVSQFVSSM
SASADVLAMSKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTKKEIDQEAAVEVSQLRDPQHDLDRVKKPEW
VILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRKGPAPLNLEVPAYEFTSDDVVVVG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025710
ORF Size 822 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025710.2
RefSeq Size 1313 bp
RefSeq ORF 825 bp
Locus ID 66694
UniProt ID Q9CR68
Cytogenetics 13 A3.2
MW 29.4 kDa
Summary Cytochrome b-c1 complex subunit Rieske, mitochondrial: Component of the mitochondrial ubiquinol-cytochrome c reductase complex dimer (complex III dimer), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis (PubMed:28673544). Incorporation of UQCRFS1 is the penultimate step in complex III assembly (By similarity).UniProtKB/Swiss-Prot Function
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"NM_025710" in other vectors (4)
SKU Description Size Price
MC204299 Uqcrfs1 (untagged) - Mouse ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 (Uqcrfs1), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$300.00
MG203679 Uqcrfs1 (tGFP-tagged) - Mouse ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 (Uqcrfs1) 10 ug
$500.00
MR203679L3 Lenti ORF clone of Uqcrfs1 (Myc-DDK-tagged) - Mouse ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 (Uqcrfs1), nuclear gene encoding mitochondrial protein 10 ug
$600.00
MR203679L4 Lenti ORF clone of Uqcrfs1 (mGFP-tagged) - Mouse ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 (Uqcrfs1), nuclear gene encoding mitochondrial protein 10 ug
$600.00

Plasmid Map for MR203679

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203679
Circular map for MR203679

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