Calb2 (NM_007586) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203611
Calb2 (Myc-DDK-tagged) - Mouse calbindin 2 (Calb2)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Calb2
Synonyms CR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203611 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGGCCCGCAGCAGCAGCCCCCTTACCTGCACCTGGCCGAGCTGACTGCATCCCAGTTCCTGGAAA
TCTGGAAGCACTTTGATGCTGACGGAAATGGGTACATTGAAGGTAAAGAGCTAGAGAACTTCTTCCAGGA
GCTGGAGAAGGCAAGGAAGGGTTCTGGCATGATGTCCAAGAGCGACAATTTTGGAGAGAAGATGAAGGAG
TTCATGCAGAAGTATGATAAGAACTCAGATGGGAAAATTGAGATGGCGGAGCTGGCGCAGATCCTGCCGA
CCGAAGAGAATTTCCTTTTGTGCTTCAGGCAGCACGTGGGCTCCAGCGCTGAGTTTATGGAGGCTTGGCG
GAAGTATGACACAGACAGAAGTGGCTACATCGAAGCCAATGAGCTCAAGGGATTCCTGTCTGACCTCCTG
AAGAAGGCCAACAGGCCCTATGATGAACCTAAGCTCCAGGAGTACACCCAGACCATACTACGGATGTTTG
ACTTAAATGGAGATGGCAAATTGGGTCTCTCAGAGATGTCTAGACTCTTGCCTGTACAAGAGAACTTCCT
GCTGAAATTTCAGGGTATGAAGCTGACCTCAGAAGAGTTCAATGCCATCTTCACATTTTATGACAAGGAT
GGAAGCGGCTATATTGATGAGAATGAACTGGACGCCCTCCTGAAGGATCTGTATGAGAAGAACAAGAAGG
AGATGAACATCCAACAGCTCACCACCTACAGGAAGAGTGTCATGTCCTTGGCCGAGGCAGGGAAGCTCTA
CAGAAAGGACCTGGAGATTGTGCTCTGCAGTGAGCCCCCCGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203611 protein sequence
Red=Cloning site Green=Tags(s)

MAGPQQQPPYLHLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKE
FMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQHVGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLL
KKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQGMKLTSEEFNAIFTFYDKD
GSGYIDENELDALLKDLYEKNKKEMNIQQLTTYRKSVMSLAEAGKLYRKDLEIVLCSEPPV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007586
ORF Size 813 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007586.2
RefSeq Size 1462 bp
RefSeq ORF 816 bp
Locus ID 12308
UniProt ID Q08331
Cytogenetics 8 D3
MW 31.4 kDa
Summary Calretinin is a calcium-binding protein which is abundant in auditory neurons.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_007586" in other vectors (5)
SKU Description Size Price
MG203611 Calb2 (tGFP-tagged) - Mouse calbindin 2 (Calb2) 10 ug
$500.00
MR203611L1 Lenti ORF clone of Calb2 (Myc-DDK-tagged) - Mouse calbindin 2 (Calb2) 10 ug
$600.00
MR203611L2 Lenti ORF clone of Calb2 (mGFP-tagged) - Mouse calbindin 2 (Calb2) 10 ug
$600.00
MR203611L3 Lenti ORF clone of Calb2 (Myc-DDK-tagged) - Mouse calbindin 2 (Calb2) 10 ug
$600.00
MR203611L4 Lenti ORF clone of Calb2 (mGFP-tagged) - Mouse calbindin 2 (Calb2) 10 ug
$600.00

Plasmid Map for MR203611

Download sequence for

Cloning scheme for MR203611

Circular map for MR203611

file-alt Citations

Investigating novel therapeutic approaches and targets to prevent synapse degeneration
Amorim
Thesis (2017)
Snippet: Page 1. This thesis has been submitted in fulfilment of the requirements for a postgraduate degree (eg PhD, MPhil, DClinPsychol) at the University of Edinburgh. Please note the following terms and conditions of use: This work ...;pCMV6-Calb2 Plasmid Origene MR203611 pCMV6-empty Plasmid Origene PS100001
Showing 1-1 of 1 papers.
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