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Tmem189 (NM_145538) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203603
Tmem189 (Myc-DDK-tagged) - Mouse transmembrane protein 189 (Tmem189)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem189
Synonyms AI840826; Kua
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203603 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGCGCCGAGGACGCGCCGGGCCGGCAGCCGGAGCTGGACGAGGACGAGACGGCGGAGGGCCGGC
GCTGGGGCGCACAGCACGCGGGAGCCCGTGAGCTGGCGGCGCTTTACTCGCCAGGCAAGCGCTTCCAGGA
GTGGTGTTCCGTGATCCTCTGTTTCAGTCTCATCGCCCACAACTTGGTCCACCTCCTGCTGCTGGCCCGC
TGGGAGCACACACCCCTCGTGATCCTGGGTGTCGTTGCAGGGGCTCTCGTCGCTGACTTCCTGTCCGGCC
TGGTACATTGGGGTGCTGATACATGGGGTTCTGTGGACCTGCCCATTGTCGGGAAGGCCTTCATCCGGCC
ATTCCGGGAGCACCACATTGACCCCACAGCCATCACAAGGCATGACTTCATCGAGACCAATGGGGACAAC
TGCCTGGTCACGCTGCTGCCACTGCTAAACATGGCCTACAAGTTCCGGACCCAGAGCCCAGAGACCCTGG
AGCAGCTGTACCCTTGGGAATGCTTCGTCTTCTGTCTGACCATCTTTGGCACCTTCACCAACCAGATCCA
CAAGTGGTCACACACATACTTAGGGCTGCCATACTGGGTCACCGTCCTGCAGGACTGGCACGTGATCCTA
CCACGGAAACACCATCGCATCCACCACGTGGCGCCCCACGAAACATACTTCTGTATCACCACAGGCTGGC
TCAACTACCCCCTAGAGGTTATAGGCTTCTGGCGGCGCCTGGAGGATCTCATCCAAGGCCTGACGGGTGA
GAAGCCGAGAGCGGACGACATGAAATGGGCACAGAAGATCAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203603 protein sequence
Red=Cloning site Green=Tags(s)

MAGAEDAPGRQPELDEDETAEGRRWGAQHAGARELAALYSPGKRFQEWCSVILCFSLIAHNLVHLLLLAR
WEHTPLVILGVVAGALVADFLSGLVHWGADTWGSVDLPIVGKAFIRPFREHHIDPTAITRHDFIETNGDN
CLVTLLPLLNMAYKFRTQSPETLEQLYPWECFVFCLTIFGTFTNQIHKWSHTYLGLPYWVTVLQDWHVIL
PRKHHRIHHVAPHETYFCITTGWLNYPLEVIGFWRRLEDLIQGLTGEKPRADDMKWAQKIK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145538
ORF Size 813 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145538.2, NP_663513.1
RefSeq Size 1700 bp
RefSeq ORF 816 bp
Locus ID 407243
UniProt ID Q99LQ7
Cytogenetics 2 H3
MW 31.1 kDa
Summary Plasmanylethanolamine desaturase involved in plasmalogen biogenesis in the endoplasmic reticulum membrane. Plasmalogens are glycerophospholipids with a hydrocarbon chain linked by a vinyl ether bond at the glycerol sn-1 position, and are involved in antioxidative and signaling mechanisms.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_145538" in other vectors (4)
SKU Description Size Price
MC200169 Tmem189 (untagged) - Mouse transmembrane protein 189 (Tmem189), (10ug) 10 ug
$300.00
MG203603 Tmem189 (tGFP-tagged) - Mouse expressed sequence AI840826 (AI840826) 10 ug
$500.00
MR203603L3 Lenti ORF clone of Tmem189 (Myc-DDK-tagged) - Mouse transmembrane protein 189 (Tmem189) 10 ug
$600.00
MR203603L4 Lenti ORF clone of Tmem189 (mGFP-tagged) - Mouse transmembrane protein 189 (Tmem189) 10 ug
$600.00

Plasmid Map for MR203603

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203603
Circular map for MR203603

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