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Gdpgp1 (NM_178752) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203595
Gdpgp1 (Myc-DDK-tagged) - Mouse RIKEN cDNA D330012F22 gene (D330012F22Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gdpgp1
Synonyms D330012F22Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203595 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGTGCCACATCATTCGCTGGAGACTTCCTATCTGCTGCCTCCAGATCCAGAGGATTGGGAGAAAC
AAGGCATCCCGGACTTTGTCTATGGCCAGGAGGATCTCGTTGGCAAGGAAGTTCAGTGGCCCAGGGATTC
ACCTAGTGCCGTGGACACAGTACCACTATCCCGTTTCGACTCTGCCCTCCGCTCTGCGTGGAGGCAGCGG
CTGGAACTGGGCCTGTTTCGGTACCGCCTAGAAGATCTGCAGACCCAAATCCTCCCCGGTTCCGTGGGGT
TTGTAGCTCAGCTGAATATAGATCGAGGGATACAGAGGAGGCGCCCCCAGAATATCAGAAGTGTGAGGCA
GGAGTTTGACCCGGAACAGTTTAACTTCAATAAAATTCGACCTGGAGAAGTCCTTTTCCGGATGCAAAGG
GAGCCTAAAGGCCCAGCCACTCCAAAGCAAGAGGACGACGTCCTTGTGGTGATCAATGTCAGCCCCCTGG
AGTGGGGTCATGTGCTGCTGGTGCCTGCACCTGCTCAGGGGCTTCCCCAACGGCTGCTGCCTGGAGTGCT
GCGGGTGGGGCTAGAAGCTGTCCTACTGAGCTTGCACCCAGGCTTCAGGGTCGGCTTCAACAGCCTGGGA
GGCCTGGCCTCTGTGAACCATCTTCACCTGCATTGCTACTACCTGGCCCACCCACTGCCTGTGGAGGGAG
CGCCAAGCACACCCCTAGACCCCAAAGGTTGTATACATCTGCTGCAGGCCCTGCCGGCTCCTGGCTTTCT
CTTTTACACCAGTGGGCCAGGGCCGGATTTGGAAGTGTTAATTAGCAGGGTATGCCGAGCTACTGACTAC
CTGAGTGACCGTGAGATTGCACATAACTTATTTGTGACCCGGGGAGCTCCCCCCGGGCCGACATCATCTA
CCTCAGACCTCAGTGGGATCCGAGTCATTCTGTGGGCCCGGAAGTCCAGCTTTGGAATAAAGGAAAGTGG
GGCGTTTAATGTTGCCCTCTGTGAGCTGGCTGGCCACCTCCCTGTCAAAACATCACAGGACTTCAGCAGC
TTGACAGAGGCAGCTGCAGTGGCCCTCATTCAGGATTGTCTGCTGCCTGAAACTCAGGCAGGTGAGGTAC
GGGCAGCACTGGTGGCCCTGATGGCCCAGGAAGAGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203595 protein sequence
Red=Cloning site Green=Tags(s)

MAVPHHSLETSYLLPPDPEDWEKQGIPDFVYGQEDLVGKEVQWPRDSPSAVDTVPLSRFDSALRSAWRQR
LELGLFRYRLEDLQTQILPGSVGFVAQLNIDRGIQRRRPQNIRSVRQEFDPEQFNFNKIRPGEVLFRMQR
EPKGPATPKQEDDVLVVINVSPLEWGHVLLVPAPAQGLPQRLLPGVLRVGLEAVLLSLHPGFRVGFNSLG
GLASVNHLHLHCYYLAHPLPVEGAPSTPLDPKGCIHLLQALPAPGFLFYTSGPGPDLEVLISRVCRATDY
LSDREIAHNLFVTRGAPPGPTSSTSDLSGIRVILWARKSSFGIKESGAFNVALCELAGHLPVKTSQDFSS
LTEAAAVALIQDCLLPETQAGEVRAALVALMAQEEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178752
ORF Size 1158 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178752.3, NP_848867.2
RefSeq Size 3520 bp
RefSeq ORF 1161 bp
Locus ID 269952
UniProt ID Q3TLS3
Cytogenetics 7 D2
MW 42.5 kDa
Summary Specific and highly efficient GDP-D-glucose phosphorylase regulating the levels of GDP-D-glucose in cells.UniProtKB/Swiss-Prot Function
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"NM_178752" in other vectors (4)
SKU Description Size Price
MC214298 Gdpgp1 (untagged) - Mouse RIKEN cDNA D330012F22 gene (D330012F22Rik), (10ug) 10 ug
$503.00
MG203595 Gdpgp1 (tGFP-tagged) - Mouse RIKEN cDNA D330012F22 gene (D330012F22Rik) 10 ug
$657.00
MR203595L3 Lenti ORF clone of Gdpgp1 (Myc-DDK-tagged) - Mouse RIKEN cDNA D330012F22 gene (D330012F22Rik) 10 ug
$757.00
MR203595L4 Lenti ORF clone of Gdpgp1 (mGFP-tagged) - Mouse RIKEN cDNA D330012F22 gene (D330012F22Rik) 10 ug
$757.00

Plasmid Map for MR203595

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203595
Circular map for MR203595

file-alt Citations

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