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Prkab1 (NM_031869) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203594
Prkab1 (Myc-DDK-tagged) - Mouse protein kinase, AMP-activated, beta 1 non-catalytic subunit (Prkab1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Prkab1
Synonyms 1300015D22Rik; AU021155; E430008F22
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203594 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAACACGAGCAGCGAGCGCGCCGCGCTGGAGCGGCAGGCGGGCCACAAGACGCCGCGGAGGGACA
GCTCCGGGGGCGCCAAGGATGGGGACAGGCCCAAGATCCTCATGGACAGCCCTGAAGACGCCGACATCTT
CCACTCCGAAGAGATCAAGGCTCCAGAGAAAGAGGAATTCCTGGCCTGGCAGCACGACCTGGAAGCGAAT
GATAAAGCCCCCGCCCAGGCCCGGCCCACCGTGTTTCGATGGACAGGGGGTGGAAAGGAAGTCTACTTGT
CTGGGTCCTTCAACAACTGGAGCAAGCTTCCCCTCACGAGAAGCCAGAATAACTTTGTAGCCATCCTGGA
CCTGCCTGAAGGAGAGCATCAGTACAAGTTCTTCGTGGATGGACAGTGGACCCATGATCCTTCTGAGCCA
ATAGTAACCAGCCAGCTTGGCACAGTTAACAACATCATTCAAGTGAAGAAAACTGACTTTGAAGTATTTG
ATGCTTTAATGGTGGATTCCCAAAAGTGCTCCGATGTGTCTGAGCTGTCCAGCTCGCCCCCAGGACCCTA
CCACCAGGAGCCTTACATGTCTAAACCAGAAGAGAGGTTCAAAGCCCCGCCCATCCTCCCGCCACACCTG
CTGCAGGTCATCTTGAACAAGGACACGGGCATCTCTTGTGACCCAGCACTGCTTCCGGAGCCCAACCACG
TCATGCTGAACCACCTCTATGCACTCTCCATCAAGGATGGAGTGATGGTGCTCAGTGCAACACACCGGTA
CAAGAAAAAGTATGTCACCACCCTCCTCTACAAGCCGATA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203594 protein sequence
Red=Cloning site Green=Tags(s)

MGNTSSERAALERQAGHKTPRRDSSGGAKDGDRPKILMDSPEDADIFHSEEIKAPEKEEFLAWQHDLEAN
DKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSQNNFVAILDLPEGEHQYKFFVDGQWTHDPSEP
IVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYMSKPEERFKAPPILPPHL
LQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_031869
ORF Size 810 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031869.2, NP_114075.1
RefSeq Size 2051 bp
RefSeq ORF 813 bp
Locus ID 19079
UniProt ID Q9R078
Cytogenetics 5 F
MW 30.3 kDa
Summary Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_031869" in other vectors (4)
SKU Description Size Price
MC207357 Prkab1 (untagged) - Mouse protein kinase, AMP-activated, beta 1 non-catalytic subunit (Prkab1), (10ug) 10 ug
$330.00
MG203594 Prkab1 (tGFP-tagged) - Mouse protein kinase, AMP-activated, beta 1 non-catalytic subunit (Prkab1) 10 ug
$500.00
MR203594L3 Lenti ORF clone of Prkab1 (Myc-DDK-tagged) - Mouse protein kinase, AMP-activated, beta 1 non-catalytic subunit (Prkab1) 10 ug
$600.00
MR203594L4 Lenti ORF clone of Prkab1 (mGFP-tagged) - Mouse protein kinase, AMP-activated, beta 1 non-catalytic subunit (Prkab1) 10 ug
$600.00

Plasmid Map for MR203594

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203594
Circular map for MR203594

file-alt Citations

AMP kinase promotes glioblastoma bioenergetics and tumour growth
Chhipa, Fan, Anderson et al
Nat. Cell Biol. (2018)
AI Summary: OriGene’s AMPKβ2 shRNA clone was used in this study to specifically knock down AMPKβ2 expression, enabling the authors to investigate the role of this AMPK subunit in glioblastoma stem cell bioenergetics and tumor growth.
Snippet: Article.;Several independent shRNA sequences were screened for each of the used human target genes, and the sequences which exhibited maximal knockdown were used for the study. Most shRNA clones (in pLKO.1 plasmid) were from the Sigma Mission RNAi shRNA library. The AMPKβ2 shRNA clone was purchased from OriGene
Showing 1-1 of 1 papers.
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