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Tk2 (NM_021028) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203589
Tk2 (Myc-DDK-tagged) - Mouse thymidine kinase 2, mitochondrial (Tk2), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tk2
Synonyms AU024611; mt-TK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203589 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCTGCGGTCGCTGCGTAGTTGGGCTGCCCGGTCCCTGCGTAGCGTGGGCCCGGGGAGCTCCGGGA
GCCCCGGGAGCCTCGATTCGGGCGCCGGGCCGCTGTGGGCACCTCGCCGCGCCTGGCCTCCGGATAAAGA
TAGAGAAAATGATAAGGAGAAAAAAGCAGTGGTTTGTATTGAGGGCAATATTGCAAGTGGGAAGACGACA
TGCCTGGAGTTCTTCTCCAATACAACAGACGTCGAGGTGTTAATGGAGCCTGTGCTCAAGTGGAGAAATG
TCCATGGCCATAACCCTCTGAGCCTCATGTACCATGATGCCAGCCGATGGGGCCTCACACTGCAGACGTA
CGTGCAGCTCACCATGCTGGACCAGCACACGCGCCCTCAGATGTCACCTGTACGGTTGATGGAAAGGTCA
ATGTACAGCGCAAGATACATTTTTGTAGAAAACCTGTATAGAAGTGGGAAGATGCCCGAGGTTGACTACG
CGATTCTGTCTGAGTGGTTTGACTGGATCGTCAGGAACATTGATGTCTCTGTTGATCTGATAGTTTATCT
GCGAACCACTCCCGAAATCTGCTACCAGAGATTAAAGATGAGGTGCCGGGAAGAGGAGAAAGTCATTCCG
ATGGAATACCTCCATGCTATTCACCGCCTCTACGAGGAGTGGCTGGTCAACGGGAGCCTCTTCCCAGCTG
CAGCCCCTGTTCTGGTGATTGAGGCTGACCACAACTTGGAGAAAATGTTAGAACTCTTTGAACAAAACCG
GGCCCGGATATTAACTCCAGAGAACTGGAAGCATGGACCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203589 protein sequence
Red=Cloning site Green=Tags(s)

MLLRSLRSWAARSLRSVGPGSSGSPGSLDSGAGPLWAPRRAWPPDKDRENDKEKKAVVCIEGNIASGKTT
CLEFFSNTTDVEVLMEPVLKWRNVHGHNPLSLMYHDASRWGLTLQTYVQLTMLDQHTRPQMSPVRLMERS
MYSARYIFVENLYRSGKMPEVDYAILSEWFDWIVRNIDVSVDLIVYLRTTPEICYQRLKMRCREEEKVIP
MEYLHAIHRLYEEWLVNGSLFPAAAPVLVIEADHNLEKMLELFEQNRARILTPENWKHGP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021028
ORF Size 810 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021028.2, NM_021028.3, NP_066356.2
RefSeq Size 2400 bp
RefSeq ORF 813 bp
Locus ID 57813
Cytogenetics 8 D3
MW 31.3 kDa
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"NM_021028" in other vectors (4)
SKU Description Size Price
MC201232 Tk2 (untagged) - Mouse thymidine kinase 2, mitochondrial (Tk2), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$300.00
MG203589 Tk2 (tGFP-tagged) - Mouse thymidine kinase 2, mitochondrial (Tk2) 10 ug
$500.00
MR203589L3 Lenti ORF clone of Tk2 (Myc-DDK-tagged) - Mouse thymidine kinase 2, mitochondrial (Tk2), nuclear gene encoding mitochondrial protein 10 ug
$600.00
MR203589L4 Lenti ORF clone of Tk2 (mGFP-tagged) - Mouse thymidine kinase 2, mitochondrial (Tk2), nuclear gene encoding mitochondrial protein 10 ug
$600.00

Plasmid Map for MR203589

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203589
Circular map for MR203589

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