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Rpp30 (NM_019428) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203560
Rpp30 (Myc-DDK-tagged) - Mouse ribonuclease P/MRP 30 subunit (human) (Rpp30)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rpp30
Synonyms Rnasep2; TSG15
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203560 representing NM_019428
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGTTCGCAGATTTGGATTTAAGAGCGGGCTCAGACCTGAAGGCTCTGCGCGGACTCGTGGAGA
CTGCTGCTCACCTGGGATACTCAGTTGTTGCCATCAATCACATTGTTGACTTTAAGGAAAAGAAACGGGA
AATTGAAAAGCCAATAGCAGTTTCTGAACTCTTCACAACTTTACCCATCGTACAGGGAAAATCAAGACCA
ATTAAAATTTTAACTAGATTGACAATTATAGTTACAGATCCAGCTCACTGTAATGTTTTGAGAGCAACTT
CGTCAAGGGTCCGGCTGTATGATATTGTTGCAGTCTTTCCAAAGACAGAAAAGCTTTTCCATGTTGCTTG
TACACATTTAGATGTGGATTTAGTCTGTATAACTGTAACAGAGAAATTACCATTTTACTTCAAAAGACCC
CCTGTTAATGTGGCAATTGAGCGAGGCCTGGGCTTTGAACTTGTCTATGGTCCTGCTATCAGAGATGCAA
CGATGAGAAGGTACACAATCTCCAATGCCCTCAATTTGATGCAGATCTGCAAAGGAAAGAATGTAATTCT
GTCCAGTGCTGCAGAAAGGCCTTTGGAAATAAGAGGACCATATGACGTGGCAAACTTAGGACTGCTGTTT
GGGCTTTCTGAAAATGATGGCAAGGCTGCCGTGTCCACAAATTGCAGAGCTGTATTTCTCCATGGAGAAA
CTAGAAAAACTGCTTTTGGAATTATTTCTACAGTGAAGAAACCACGGCCATCAGAAGCAGATGATGAATC
TCTTCCAGTGTGCAAGAAAGCTAAATGTGAGGGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203560 representing NM_019428
Red=Cloning site Green=Tags(s)

MAAFADLDLRAGSDLKALRGLVETAAHLGYSVVAINHIVDFKEKKREIEKPIAVSELFTTLPIVQGKSRP
IKILTRLTIIVTDPAHCNVLRATSSRVRLYDIVAVFPKTEKLFHVACTHLDVDLVCITVTEKLPFYFKRP
PVNVAIERGLGFELVYGPAIRDATMRRYTISNALNLMQICKGKNVILSSAAERPLEIRGPYDVANLGLLF
GLSENDGKAAVSTNCRAVFLHGETRKTAFGIISTVKKPRPSEADDESLPVCKKAKCEG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019428
ORF Size 804 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019428.3, NP_062301.3
RefSeq Size 1172 bp
RefSeq ORF 807 bp
Locus ID 54364
UniProt ID O88796
Cytogenetics 19 C2
MW 29.5 kDa
Summary Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.UniProtKB/Swiss-Prot Function
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"NM_019428" in other vectors (4)
SKU Description Size Price
MC209966 Rpp30 (untagged) - Mouse ribonuclease P/MRP 30 subunit (human) (Rpp30), (10ug) 10 ug
$330.00
MG203560 Rpp30 (tGFP-tagged) - Mouse ribonuclease P/MRP 30 subunit (human) (Rpp30) 10 ug
$500.00
MR203560L3 Lenti ORF clone of Rpp30 (Myc-DDK-tagged) - Mouse ribonuclease P/MRP 30 subunit (human) (Rpp30) 10 ug
$600.00
MR203560L4 Lenti ORF clone of Rpp30 (mGFP-tagged) - Mouse ribonuclease P/MRP 30 subunit (human) (Rpp30) 10 ug
$600.00

Plasmid Map for MR203560

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203560
Circular map for MR203560

file-alt Citations

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