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Tssk3 (NM_080442) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203558
Tssk3 (Myc-DDK-tagged) - Mouse testis-specific serine kinase 3 (Tssk3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tssk3
Synonyms 1700014N07Rik; 4930594I21Rik; Stk22c; Stk22d; Tsk-3; Tssk-3; Tssk3b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203558 representing NM_080442
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGACTTTCTACTCTCCAATGGGTATCAGCTGGGCAAGACCATTGGGGAAGGGACCTACTCAAAAG
TCAAAGAAGCATTTTCCAAAAAACATCAAAGAAAAGTGGCAATTAAAATTATAGACAAGATGGGAGGGCC
AGAAGAGTTTATCCAGAGATTCCTGCCTCGTGAGCTCCAGATTGTCCGTACCCTGGACCACAAAAACATC
ATCCAGGTGTATGAGATGCTGGAGTCAGCAGATGGAAAAATCTACCTGGTGATGGAACTGGCTGAGGGAG
GGGATGTCTTTGACTGTGTGCTGAACGGAGGGCCACTTCCCGAGAGCCGGGCCAAGGCCCTCTTCCGCCA
GATGGTTGAGGCTATTCGCTATTGCCATGGCTGTGGCGTGGCCCACCGGGACCTTAAGTGTGAGAACGCC
TTGTTGCAGGGCTTCAACCTGAAGCTGACCGACTTTGGCTTTGCCAAGGTGCTACCCAAGTCACGCAGGG
AGCTGAGCCAGACCTTCTGTGGCAGCACAGCCTATGCCGCCCCTGAGGTGCTACAGGGCATACCCCATGA
TAGCAAGAAAGGTGATGTCTGGAGCATGGGTGTGGTCCTGTATGTAATGCTCTGTGCAAGTCTACCTTTT
GATGACACAGATATCCCCAAGATGCTGTGGCAGCAGCAGAAGGGGGTGTCCTTCCCCACTCATTTGGGCA
TCTCAACCGAATGCCAGGACCTGCTCAAGCGGCTCCTGGAACCAGACATGATACTCCGGCCTTCAATCGA
AGAAGTTAGTTGGCACCCATGGCTAGCAAGCACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203558 representing NM_080442
Red=Cloning site Green=Tags(s)

MEDFLLSNGYQLGKTIGEGTYSKVKEAFSKKHQRKVAIKIIDKMGGPEEFIQRFLPRELQIVRTLDHKNI
IQVYEMLESADGKIYLVMELAEGGDVFDCVLNGGPLPESRAKALFRQMVEAIRYCHGCGVAHRDLKCENA
LLQGFNLKLTDFGFAKVLPKSRRELSQTFCGSTAYAAPEVLQGIPHDSKKGDVWSMGVVLYVMLCASLPF
DDTDIPKMLWQQQKGVSFPTHLGISTECQDLLKRLLEPDMILRPSIEEVSWHPWLAST

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080442
ORF Size 804 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080442.2, NP_536690.1
RefSeq Size 1034 bp
RefSeq ORF 807 bp
Locus ID 58864
UniProt ID Q9D2E1
Cytogenetics 4 D2.2
MW 30.7 kDa
Summary May be involved in a signaling pathway during male germ cell development or mature sperm function.UniProtKB/Swiss-Prot Function
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Documents
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"NM_080442" in other vectors (4)
SKU Description Size Price
MC205051 Tssk3 (untagged) - Mouse testis-specific serine kinase 3 (Tssk3), (10ug) 10 ug
$300.00
MG203558 Tssk3 (tGFP-tagged) - Mouse testis-specific serine kinase 3 (Tssk3) 10 ug
$500.00
MR203558L3 Lenti ORF clone of Tssk3 (Myc-DDK-tagged) - Mouse testis-specific serine kinase 3 (Tssk3) 10 ug
$600.00
MR203558L4 Lenti ORF clone of Tssk3 (mGFP-tagged) - Mouse testis-specific serine kinase 3 (Tssk3) 10 ug
$600.00

Plasmid Map for MR203558

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203558
Circular map for MR203558

file-alt Citations

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