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Zdhhc21 (NM_026647) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203515
Zdhhc21 (Myc-DDK-tagged) - Mouse zinc finger, DHHC domain containing 21 (Zdhhc21)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Zdhhc21
Synonyms 9130404H11Rik; AL024349; D130004H04Rik; dep
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203515 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTCTTCGGATTCACTTTGTTGTTGACCCGCATGGTTGGTGCTGCATGGGCTTGATTGTCTTTGTCT
GGTTATACAATATTGTTATAATTCCCAAAATTGTCCTCTTTCCTCACTATGAAGAAGGACATATTCCTGG
CATTTTAATAATAATATTCTATGGCATTTCCATATTTTGCCTGGTTGCCTTAGTGAGGGCCTCCTTAACT
GATCCTGGAAGACTCCCTGAAAATCCCAAGATCCCACATGCAGAGCGGGAGCTGTGGGAGCTCTGCAACA
AGTGCAACCTGATGAGGCCGAAGCGCTCCCACCACTGCAGCCGCTGCGGCCACTGCGTCAGGAGGATGGA
CCATCACTGTCCTTGGATAAACAATTGTGTGGGTGAAGACAACCATTGGCTCTTTCTGCAGTTGTGTTTC
TACACTGAGCTGCTTACTTGCTACGCACTGATGTTTTCTTTCTGCCATTATTATTATTTTCTTCCACTAA
AAAAGCGTAACTTGGATCTCTTTGTTGTTCGCCATGAGCTGGCTATCATGAGACTAGCTGCCTTCATGGG
CATCACTATGTTAGTTGGAATAACTGGACTCTTCTATACACAACTGATTGGCATCATCACAGATACAACA
TCTATTGAAAAAATGTCAAATTGTTGTGAAGAAATATCGCGGCCCCGAAAGCCATGGCAGCAGACCTTCT
CAGAAGTTTTTGGCACTCGTTGGAAGATCCTGTGGTTCATTCCTTTCAGGCAGCGGCAACCACTGCGAGT
TCCCTACCACTTTGCCAATCACGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203515 protein sequence
Red=Cloning site Green=Tags(s)

MGLRIHFVVDPHGWCCMGLIVFVWLYNIVIIPKIVLFPHYEEGHIPGILIIIFYGISIFCLVALVRASLT
DPGRLPENPKIPHAERELWELCNKCNLMRPKRSHHCSRCGHCVRRMDHHCPWINNCVGEDNHWLFLQLCF
YTELLTCYALMFSFCHYYYFLPLKKRNLDLFVVRHELAIMRLAAFMGITMLVGITGLFYTQLIGIITDTT
SIEKMSNCCEEISRPRKPWQQTFSEVFGTRWKILWFIPFRQRQPLRVPYHFANHV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026647
ORF Size 795 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026647.4
RefSeq Size 8751 bp
RefSeq ORF 798 bp
Locus ID 68268
UniProt ID Q9D270
Cytogenetics 4 C3
MW 31.3 kDa
Summary Palmitoylates sex steroid hormone receptors, including ESR1, PGR and AR, thereby regulating their targeting to the plasma membrane. This affects rapid intracellular signaling by sex hormones via ERK and AKT kinases and the generation of cAMP, but does not affect that mediated by their nuclear receptor (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026647" in other vectors (4)
SKU Description Size Price
MC202578 Zdhhc21 (untagged) - Mouse zinc finger, DHHC domain containing 21 (Zdhhc21), (10ug) 10 ug
$300.00
MG203515 Zdhhc21 (tGFP-tagged) - Mouse zinc finger, DHHC domain containing 21 (Zdhhc21) 10 ug
$500.00
MR203515L3 Lenti ORF clone of Zdhhc21 (Myc-DDK-tagged) - Mouse zinc finger, DHHC domain containing 21 (Zdhhc21) 10 ug
$600.00
MR203515L4 Lenti ORF clone of Zdhhc21 (mGFP-tagged) - Mouse zinc finger, DHHC domain containing 21 (Zdhhc21) 10 ug
$600.00

Plasmid Map for MR203515

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203515
Circular map for MR203515

file-alt Citations

Palmitoyl acyltransferase DHHC21 mediates endothelial dysfunction in systemic inflammatory response syndrome
Beard, Yang, Meegan et al
Nat Commun (2016) 7, 12823 DOI: 10.1038/ncomms12823
Cited by 83
AI Summary: OriGene contributed pCMV6 empty vector and ZDHHC21 plasmids, which were used for overexpression studies to investigate the role of DHHC21 in endothelial inflammation in this paper.
Snippet: All Zdhhc and PLCβ1 siRNA were purchased from Santa Cruz Biotech. pCMV6 empty vector, and ZDHHC21 plasmids were purchased from Origene;and pLX304 empty vector and PLCβ1 plasmids were purchased from Genecopoeia
Showing 1-1 of 1 papers.
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