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Olah (NM_145921) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203507
Olah (Myc-DDK-tagged) - Mouse oleoyl-ACP hydrolase (Olah)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Olah or NM_145921"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Olah
Synonyms AI607300; E230009B14Rik; Thedc1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203507 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACAGCAGTCTATGCTAAGAGTACCAGGAATGAGAAGGTTTTGAACTGTTTGTGTCAAAAGCCGG
ATGCACTTTTCAAGCTGATCTGCTTTCCCTGGGCAGGAGGCGGCTCCACTCATTTTGCCAAGTGGGGCCG
AAAGATTAATGGCTTATTGGAAGTGCATGCTGTAAGACTGGCTGGGAGAGAAACCCGATTTGAAGAACCT
TTCTCAAATGACATCTACCAGATAGCTGAGGAAGTTGTGACTGCCCTGTTGCCCATCATCCGTGACAAAG
CTTTTGCATTTTTTGGCCACAGTTTCGGATCCTACATTGCTTTTATTACTGCACTGCACCTAAAGGAGAA
ATATAAAATGGAGCCGCTGCATATCTTTGTGTCCAGTGCCTCTGCCCCTCACTCAGAATTCCGGCCTCAA
GTTCCTGATATTAACAAATTGTCAGAAGAACAAATAAGAGATCACCTTCTGATTTTTGGAGGCACTCCCA
AGCATCTCATCGAAGACCAGGATTTTCTGAAGCAATGCATTCCTTTGCTGAAGGCAGATGTTGACATTGT
GAAAAAATTCATCTTTGACAAACCCTCCAAAGCTCTCCTTTCTCGGGACATCACGTGCTTTATTGGATCT
GAAGATGTAGTAAAGGATATAGAAGGCTGGAAAGACATAACCAGTGGGAAGTTTGATGTCCTCAAGCTGC
CAGGTGACCACTTTTATCTGATGGAACCGAACAACGAGGACTTTATCAAGAACTACATAGTCAAGTGTTT
GGAGCTCTCCTCACTTGACTACTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203507 protein sequence
Red=Cloning site Green=Tags(s)

METAVYAKSTRNEKVLNCLCQKPDALFKLICFPWAGGGSTHFAKWGRKINGLLEVHAVRLAGRETRFEEP
FSNDIYQIAEEVVTALLPIIRDKAFAFFGHSFGSYIAFITALHLKEKYKMEPLHIFVSSASAPHSEFRPQ
VPDINKLSEEQIRDHLLIFGGTPKHLIEDQDFLKQCIPLLKADVDIVKKFIFDKPSKALLSRDITCFIGS
EDVVKDIEGWKDITSGKFDVLKLPGDHFYLMEPNNEDFIKNYIVKCLELSSLDYF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145921
ORF Size 795 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145921.1, NP_666033.1
RefSeq Size 1522 bp
RefSeq ORF 798 bp
Locus ID 99035
UniProt ID Q8R197
Cytogenetics 2 A1
MW 30.2 kDa
Summary Contributes to the release of free fatty acids from fatty acid synthase (FASN). Has broad substrate specificity, giving rise to a range of free fatty acids with chain lengths between 10 and 16 carbon atoms (C10 - C16).UniProtKB/Swiss-Prot Function
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Documents
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"NM_145921" in other vectors (4)
SKU Description Size Price
MC201666 Olah (untagged) - Mouse oleoyl-ACP hydrolase (Olah), (10ug) 10 ug
$300.00
MG203507 Olah (tGFP-tagged) - Mouse oleoyl-ACP hydrolase (Olah) 10 ug
$500.00
MR203507L3 Lenti ORF clone of Olah (Myc-DDK-tagged) - Mouse oleoyl-ACP hydrolase (Olah) 10 ug
$600.00
MR203507L4 Lenti ORF clone of Olah (mGFP-tagged) - Mouse oleoyl-ACP hydrolase (Olah) 10 ug
$600.00

Plasmid Map for MR203507

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203507
Circular map for MR203507

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