Pik3ip1 (NM_178149) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203501
Pik3ip1 (Myc-DDK-tagged) - Mouse phosphoinositide-3-kinase interacting protein 1 (Pik3ip1)
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pik3ip1
Synonyms 1500004A08Rik; 5830455E04Rik; Crkd; Hgfl
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203501 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTTGGCTTGGGTACACACATTTCTTCTCAGCAACATGCTTCTGGCAGAAGCCTATGGATCTGGAG
GCTGCTTCTGGGACAACGGCCACCTGTACCGGGAGGACCAGCCCTCGCCCGCGCCGGGTCTCCGCTGCCT
CAACTGGTTGGCCGCGCAAGGCAGCCGCGAGTCGCTCACCGAGCCCAGCCCGGGCAACCACAACTACTGC
CGGAACCCGGACCAGGACCCGCGCGGGCCCTGGTGCTACATCAGCAGCGAGACCGGCGTCCCTGAAAAGC
GGCCCTGCGAGGACGTGAGTTGCCCAGAGACCACTTCCCAAGCACCACCGCCATCCTCTGCCATGGAGCT
GGAAGAGAAGTCTGGTGCACCAGGTGACAAAGAGGCACAGGTGTTCCCTCCTGCTAACGCCCTACCAGCC
AGGAGTGAGGCAGCCGAGGTGCAGCCAGTGATCGGGATCAGTCAGCTTGTGAGGATGAACTCCAAGGAAA
AAAAAGACCTAGGAACTCTGGGCTACGTGCTGGGCATTACTATGATGGTGATCATCCTCGCTATTGGAGC
TGGCATTATCGTGGGCTACACTTACAAGAGGGGGAAGGACTTGAAAGAGCAACATGAGAAGAAAGCGTGT
GAGAGGGAGATGCAGCGAATCACCTTGCCCCTGTCTGCTTTCACAAACCCCACCTGTGAGACCGTGGATG
AAAACACCATCATTGTGCACAGCAACCAGACTCCTGCTGACGTGCAGGAGGGCAGCACCCTCCTCACGGG
CCAGGCTGGCACCCCTGGGGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203501 protein sequence
Red=Cloning site Green=Tags(s)

MLLAWVHTFLLSNMLLAEAYGSGGCFWDNGHLYREDQPSPAPGLRCLNWLAAQGSRESLTEPSPGNHNYC
RNPDQDPRGPWCYISSETGVPEKRPCEDVSCPETTSQAPPPSSAMELEEKSGAPGDKEAQVFPPANALPA
RSEAAEVQPVIGISQLVRMNSKEKKDLGTLGYVLGITMMVIILAIGAGIIVGYTYKRGKDLKEQHEKKAC
EREMQRITLPLSAFTNPTCETVDENTIIVHSNQTPADVQEGSTLLTGQAGTPGA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178149
ORF Size 792 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178149.2, NM_178149.3, NM_178149.4, NP_835362.2
RefSeq Size 2267 bp
RefSeq ORF 795 bp
Locus ID 216505
UniProt ID Q7TMJ8
Cytogenetics 11 A1
MW 28.6 kDa
Summary Negative regulator of hepatic phosphatidylinositol 3-kinase (PI3K) activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178149" in other vectors (3)
SKU Description Size Price
MG203501 Pik3ip1 (tGFP-tagged) - Mouse RIKEN cDNA 1500004A08 gene (1500004A08Rik) 10 ug
$650.00
MR203501L3 Lenti ORF clone of Pik3ip1 (Myc-DDK-tagged) - Mouse phosphoinositide-3-kinase interacting protein 1 (Pik3ip1) 10 ug
$750.00
MR203501L4 Lenti ORF clone of Pik3ip1 (mGFP-tagged) - Mouse phosphoinositide-3-kinase interacting protein 1 (Pik3ip1) 10 ug
$750.00

Plasmid Map for MR203501

Download sequence for

Cloning scheme for MR203501

Circular map for MR203501

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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