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Nnmt (NM_010924) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203499
Nnmt (Myc-DDK-tagged) - Mouse nicotinamide N-methyltransferase (Nnmt)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nnmt
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203499 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAATCTGGCTTCACCTCCAAGGACACTTATCTAAGTCATTTTAATCCTCGGGATTACTTGGAAAAAT
ATTACAGCTTTGGGTCCAGACACTGTGCAGAAAATGAGATCCTCAGACATCTGCTGAAAAATCTTTTTAA
GATATTCTGCCTGGGTGCTGTAAAGGGAGAACTCCTGATTGACATTGGCTCTGGCCCCACCATCTATCAG
CTTCTCTCTGCCTGTGAGTCCTTCACGGAGATCATTGTCTCTGACTACACAGACCAGAACCTCTGGGAGC
TGCAGAAATGGCTGAAGAAGGAACCAGGAGCCTTTGACTGGTCCCCAGTGGTCACCTATGTGTGTGATCT
TGAAGGCAACAGAATGAAGGGACCTGAGAAGGAGGAGAAGTTGAGGCGTGCAATCAAGCAGGTACTAAAG
TGTGATGTGACCCAGAGCCAGCCTCTGGGTGGGGTCTCTCTGCCTCCTGCTGACTGCCTGCTCAGCACAC
TGTGCCTGGATGCTGCCTGCCCTGACCTCCCAGCCTATCGCACTGCCCTCAGGAACCTGGGCAGTCTGCT
CAAGCCAGGAGGCTTCCTGGTGATGGTAGATGCCCTGAAGAGTAGCTACTACATGATTGGGGAGCAGAAG
TTTTCCAGCCTTCCCCTGGGATGGGAGACTGTTCGAGATGCTGTGGAAGAGGCCGGTTACACCATTGAGC
AATTTGAGGTGATTTCTCAAAATTACTCTTCTACCACATCCAATAATGAAGGACTCTTCTCCCTAGTGGG
GCGAAAGCCAGGCAGATCTGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203499 protein sequence
Red=Cloning site Green=Tags(s)

MESGFTSKDTYLSHFNPRDYLEKYYSFGSRHCAENEILRHLLKNLFKIFCLGAVKGELLIDIGSGPTIYQ
LLSACESFTEIIVSDYTDQNLWELQKWLKKEPGAFDWSPVVTYVCDLEGNRMKGPEKEEKLRRAIKQVLK
CDVTQSQPLGGVSLPPADCLLSTLCLDAACPDLPAYRTALRNLGSLLKPGGFLVMVDALKSSYYMIGEQK
FSSLPLGWETVRDAVEEAGYTIEQFEVISQNYSSTTSNNEGLFSLVGRKPGRSE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010924
ORF Size 792 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010924.1, NM_010924.2, NM_010924.3, NP_035054.1
RefSeq Size 954 bp
RefSeq ORF 795 bp
Locus ID 18113
UniProt ID O55239
Cytogenetics 9 26.45 cM
MW 29.6 kDa
Summary Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.UniProtKB/Swiss-Prot Function
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"NM_010924" in other vectors (3)
SKU Description Size Price
MG203499 Nnmt (tGFP-tagged) - Mouse nicotinamide N-methyltransferase (Nnmt) 10 ug
$489.00 $500.00
MR203499L3 Lenti ORF clone of Nnmt (Myc-DDK-tagged) - Mouse nicotinamide N-methyltransferase (Nnmt) 10 ug
$600.00
MR203499L4 Lenti ORF clone of Nnmt (mGFP-tagged) - Mouse nicotinamide N-methyltransferase (Nnmt) 10 ug
$600.00

Plasmid Map for MR203499

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203499
Circular map for MR203499

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