Mfap4 (NM_029568) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203326
Mfap4 (Myc-DDK-tagged) - Mouse microfibrillar-associated protein 4 (Mfap4)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mfap4
Synonyms 1110007F23Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203326 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGCCCTCCCAGCCCTGCCACTGATGCTGATGCTGCTCTCCATGCCTCCCCCCTGCGCCCCGCAAG
CCTCTGGGATCCGGGGAGATGCTCTGGAGAAGTCCTGTCTTCAGCAACCCCTGGACTGTGATGATATCTA
CGCCCAGGGCTATCAGGAAGACGGCGTGTATCTCATCTACCCCTATGGCCCCAGTGTGCCGGTGCCCGTC
TTCTGCGACATGACAACTGAGGGCGGCAAGTGGACGGTTTTCCAGAAAAGATTCAACGGCTCAGTGAGTT
TCTTCCGGGGCTGGAGCGACTACAAGCTGGGCTTTGGCCGTGCTGACGGGGAGTACTGGCTGGGGCTGCA
GAACCTGCACCTCCTGACACTGAAGCAGAAGTATGAGCTGCGCGTGGACTTGGAAGACTTTGAGAACAAC
ACAGCCTATGCCAAGTACATTGACTTCTCCATCTCCCCCAACGCCATCAGTGCTGAGGAGGATGGCTATA
CCCTCTACGTGGCTGGCTTCGAGGATGGCGGGGCAGGTGACTCACTGTCCTACCACAGTGGCCAGAAGTT
CTCCACCTTTGATCGGGACCAGGACCTCTTCGTGCAGAACTGTGCAGCCCTCTCCTCAGGAGCCTTCTGG
TTCCGAAGCTGCCATTTCGCCAATCTCAACGGTTTCTACCTGGGTGGTTCCCATCTCTCCTATGCCAATG
GCATCAATTGGGCCCAATGGAAAGGCTTCTATTACTCCCTCAAGCGCACGGAGATGAAAATTCGTCGGGC
C


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203326 protein sequence
Red=Cloning site Green=Tags(s)

MKALPALPLMLMLLSMPPPCAPQASGIRGDALEKSCLQQPLDCDDIYAQGYQEDGVYLIYPYGPSVPVPV
FCDMTTEGGKWTVFQKRFNGSVSFFRGWSDYKLGFGRADGEYWLGLQNLHLLTLKQKYELRVDLEDFENN
TAYAKYIDFSISPNAISAEEDGYTLYVAGFEDGGAGDSLSYHSGQKFSTFDRDQDLFVQNCAALSSGAFW
FRSCHFANLNGFYLGGSHLSYANGINWAQWKGFYYSLKRTEMKIRRA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029568
ORF Size 771 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029568.3
RefSeq Size 1513 bp
RefSeq ORF 774 bp
Locus ID 76293
UniProt ID Q9D1H9
Cytogenetics 11 37.96 cM
MW 28.9 kDa
Summary Could be involved in calcium-dependent cell adhesion or intercellular interactions. May contribute to the elastic fiber assembly and/or maintenance.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_029568" in other vectors (4)
SKU Description Size Price
MC204102 Mfap4 (untagged) - Mouse microfibrillar-associated protein 4 (Mfap4), (10ug) 10 ug
$300.00
MG203326 Mfap4 (tGFP-tagged) - Mouse microfibrillar-associated protein 4 (Mfap4) 10 ug
$489.00 $500.00
MR203326L3 Lenti ORF clone of Mfap4 (Myc-DDK-tagged) - Mouse microfibrillar-associated protein 4 (Mfap4) 10 ug
$600.00
MR203326L4 Lenti ORF clone of Mfap4 (mGFP-tagged) - Mouse microfibrillar-associated protein 4 (Mfap4) 10 ug
$600.00

Plasmid Map for MR203326

Download sequence for

Cloning scheme for MR203326

Circular map for MR203326

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