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Slfn8 (BC024709) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203303
Slfn8 (Myc-DDK-tagged) - Mouse schlafen 8 (cDNA clone MGC:30719 IMAGE:3982223)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slfn8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203303 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGGTTACTTTGAGACAAACAGGATCCAACACATCATTGTTGATGAAGCCCAGAATTTCCACACTG
AGGATGGAAACTGGTGTGAGAAGGCAAAAGGAATCACTCAAGGAGGGAAAGCTTGTCCTAGAATGTTCTG
GATATTTCTGGACTATTTTCAGACCAGTCATTTGAAGGAGAGTGGCCTCCCAGATTTCGAACTCCAGTAT
CCAAAGGAAGAGCTCACACAAGTGGTACGCAATGGAGATAAAATAGCTGAGTTCCTACAACAAGAGTTGG
GAAAAATCAGAGATAACCCTCCATGCAGCATCCCCCGAGCATCCCTAAACATTGTCCATGAATTTAAGTG
GTCCCAAAGTATATCAGGCACCCTTAAGATTAAATACTTGACTTCGGAAAAGATGGTAAGCTATGTAGCA
AATAAGTGTGATGTTTTCTTGAGTAAAGGCTATTCACCCCAAGATATTGCAGTGCTTTTCAGCACAGACA
GGGAAAAGAAAGCCTATGAGCATATGTTCCTGAGAGAAATGAGGAAGAAGAGGAGAGCATCTCAGATGAA
TGACACGTCTGTCTGTCATTCTAACATGTTTGACAGAATCCGTCGATTCTCAGGACTGGAAAGAAGCATT
GTGTTTGGTATCAATCCCATTGCAACTGAGCAGCCCATTTCCCACAACCTATTGATCTGCCTGGCTTCCA
GAGCAATGAAATATCTGTATATCCTGTATGCTTTAACTCCTGAGGGGCAGAGCTCAACGGTGGCATGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203303 protein sequence
Red=Cloning site Green=Tags(s)

MKGYFETNRIQHIIVDEAQNFHTEDGNWCEKAKGITQGGKACPRMFWIFLDYFQTSHLKESGLPDFELQY
PKEELTQVVRNGDKIAEFLQQELGKIRDNPPCSIPRASLNIVHEFKWSQSISGTLKIKYLTSEKMVSYVA
NKCDVFLSKGYSPQDIAVLFSTDREKKAYEHMFLREMRKKRRASQMNDTSVCHSNMFDRIRRFSGLERSI
VFGINPIATEQPISHNLLICLASRAMKYLYILYALTPEGQSSTVAC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC024709
ORF Size 768 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC024709, AAH24709
RefSeq Size 1859 bp
RefSeq ORF 770 bp
Locus ID 276950
Cytogenetics 11 C
MW 68.1 kDa
Summary Endoribonuclease that cleaves tRNAs and rRNAs (PubMed:29563550). Cleaves tRNAs 11 nucleotides from the 3'-terminus at the acceptor stem (PubMed:29563550). May be involved in immune system via regulation of inflammation (PubMed:29528433).UniProtKB/Swiss-Prot Function
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"BC024709" in other vectors (4)
SKU Description Size Price
MC218673 Slfn8 (untagged) - Mouse schlafen 8 (cDNA clone MGC:30719 IMAGE:3982223), (10ug) 10 ug
$330.00
MG203303 Slfn8 (tGFP-tagged) - Mouse schlafen 8 (cDNA clone MGC:30719 IMAGE:3982223) 10 ug
$500.00
MR203303L3 Lenti ORF clone of Slfn8 (Myc-DDK-tagged) - Mouse schlafen 8 (cDNA clone MGC:30719 IMAGE:3982223) 10 ug
$600.00
MR203303L4 Lenti ORF clone of Slfn8 (mGFP-tagged) - Mouse schlafen 8 (cDNA clone MGC:30719 IMAGE:3982223) 10 ug
$600.00

Plasmid Map for MR203303

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203303
Circular map for MR203303

file-alt Citations

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