Srd5a1 (NM_175283) Mouse Tagged ORF Clone
$289.00
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Srd5a1 |
| Synonyms | 0610031P22Rik; 4930435F02Rik; S5AR 1; Srd5a-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_175283 |
| ORF Size | 765 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_175283.3, NP_780492.2 |
| RefSeq Size | 2681 bp |
| RefSeq ORF | 768 bp |
| Locus ID | 78925 |
| UniProt ID | Q68FF9 |
| Cytogenetics | 13 35.55 cM |
| MW | 29.4 kDa |
| Summary | Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_175283" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC211909 | Srd5a1 (untagged) - Mouse steroid 5 alpha-reductase 1 (Srd5a1), (10ug) | 10 ug |
$330.00
|
|
| MG203282 | Srd5a1 (tGFP-tagged) - Mouse steroid 5 alpha-reductase 1 (Srd5a1) | 10 ug |
$500.00
|
|
| MR203282L3 | Lenti ORF clone of Srd5a1 (Myc-DDK-tagged) - Mouse steroid 5 alpha-reductase 1 (Srd5a1) | 10 ug |
$600.00
|
|
| MR203282L4 | Lenti ORF clone of Srd5a1 (mGFP-tagged) - Mouse steroid 5 alpha-reductase 1 (Srd5a1) | 10 ug |
$600.00
|
|
Citations
Impaired endogenous neurosteroid signaling contributes to behavioral deficits associated with chronic stress
Walton, Antonoudiou, Barros et al
bioRxiv (2022)
Walton, Antonoudiou, Barros et al
bioRxiv (2022)
Cited by 7
Showing 1-1 of 1 papers.
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