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Stk19 (NM_019442) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203265
Stk19 (Myc-DDK-tagged) - Mouse serine/threonine kinase 19 (Stk19)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Stk19
Synonyms G11; RP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203265 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCGCAAGAGACGGCTCCTGGCGTCTGAGGCTTTTGGGGTGAAGCGGAGGCGGGCGCCGGGGCCCG
TGAGAGCGGATCCTTTAAGAACAAGAGCAGGCTCGGCGCGCGAGGCCATCGAGGAGCTCGTGAAGCTGTT
TCCCCGGGGGCTGTTCGAGGATGCACTGCCGCCAATCGCGCTGAGGAGCCAAGTGTACAGTCTGGTGCCA
GACCGGACGGTGGCCGACCTACAACTGAAGGAGCTTCAAGAACTGGGAGAGATCCGAATTATCCAGCTAG
GCTTTGACTTGGATGCTCATGGGATTGTCTTCACGGAAGACTACAGGACCCGGGTCCTCAAGGCCTGTGA
TGGCCGACCATGTGCTGGGGCGGTGCAGAAGTTCCTGGCCTCAGTGCTCCCAGCCTGTGGGGACCTCAGT
TTCCAGCAGGATCAGATGACACAGACTTATGGCTTCAGGGACCCGGAGATCACGCAGCTGGTGAACGCTG
GGGTCCTCACTGTCCGAGATGCTGGAAGCTGGTGGCTGGCTGTGCCTGGAGCTGGGAGATTCATCAAGTG
CTTTGTTAAAGGGCGCCAGGCTGTACTGAGCATGGTGCGGAAGGCCAAGTACCGGGAGCTTGCCTTGTCA
GAGCTCCTGGGCCGCAGGGCCCCTTTGGCAGTGCGGCTAGGTCTTGCCTACCATGTGCACGACCTCATTG
GAGCCCAGCTGGTGGACTGTGTCCCCACCACTTCTGGAACCCTCCTTCGCCTGCCAGATACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203265 protein sequence
Red=Cloning site Green=Tags(s)

MNRKRRLLASEAFGVKRRRAPGPVRADPLRTRAGSAREAIEELVKLFPRGLFEDALPPIALRSQVYSLVP
DRTVADLQLKELQELGEIRIIQLGFDLDAHGIVFTEDYRTRVLKACDGRPCAGAVQKFLASVLPACGDLS
FQQDQMTQTYGFRDPEITQLVNAGVLTVRDAGSWWLAVPGAGRFIKCFVKGRQAVLSMVRKAKYRELALS
ELLGRRAPLAVRLGLAYHVHDLIGAQLVDCVPTTSGTLLRLPDT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019442
ORF Size 762 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019442.2
RefSeq Size 1078 bp
RefSeq ORF 765 bp
Locus ID 54402
UniProt ID Q9JHN8
Cytogenetics 17 B1
MW 28.1 kDa
Summary Serine/threonine-protein kinase that acts as a key regulator of NRAS signaling by mediating phosphorylation of NRAS at 'Ser-89', thereby enhancing NRAS-binding to its downstream effectors.UniProtKB/Swiss-Prot Function
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"NM_019442" in other vectors (3)
SKU Description Size Price
MG203265 Stk19 (tGFP-tagged) - Mouse serine/threonine kinase 19 (Stk19) 10 ug
$500.00
MR203265L3 Lenti ORF clone of Stk19 (Myc-DDK-tagged) - Mouse serine/threonine kinase 19 (Stk19) 10 ug
$600.00
MR203265L4 Lenti ORF clone of Stk19 (mGFP-tagged) - Mouse serine/threonine kinase 19 (Stk19) 10 ug
$600.00

Plasmid Map for MR203265

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203265
Circular map for MR203265

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