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Dnajc8 (BC066222) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203227
Dnajc8 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 8 (cDNA clone MGC:76784 IMAGE:30459247)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dnajc8
Synonyms 1110021D09Rik; 2010009J04Rik; AL024084; AU019262; AU044514
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203227 representing BC066222
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTTCGGGAGAGAGTGGTGCTTCGGGTGGCGGAGGCAGCACAGAGGAGGCATTTATGACCTTCT
ACAGTGAGGTGAAACAAATAGAGAAGAGAGATTCAGTTCTAACATCCAAAAATCAAATTGAAAGATTGAC
TCGTCCTGGTTCCTCTTACTTTAATCTGAACCCATTTGAGGTTCTTCAGATAGATCCTGAAGTGACAGAT
GAAGAAATAAAAAAGAGGTTTCGTCAGTTGTCCATCCTGGTGCATCCTGACAAAAACCAGGACGATGCTG
ACAGAGCACAGAAGGCTTTTGAAGCTGTGGACAAAGCTTACAAGTTGCTACTGGATCAGGAACAAAAGAA
GAGGGCCCTGGATGTAATTCAGGCAGGAAAAGAATACGTGGAACACACTGTTAAAGAGCGAAAAAAACAA
CTGAAGAAGGAAGGAAAGCCTACAAATGTGGAGGAAGATGATCCCGAACTGTTCAAACAGGCAGTCTACA
AACAGACCATGAAGCTGTTTGCTGAGCTGGAGATTAAAAGGAAAGAGAGAGAAGCCAAAGAAATGCATGA
GAGGAAGCGACAGAGAGAAGAGGAGATCGAAGCCCAGGAAAAAGCCAAGCGAGAAAGAGAGTGGCAGAAA
AACTTTGAGGAAAGTCGGGATGGTCGAGTGGACAGTTGGCGAAATTTCCAGGCAAATACAAAGGGGAAGA
AGGAGAAGAAGAATCGGACCTTCCTGCGGCCACCGAAAGTCAAGATGGAGCAGCGGGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203227 representing BC066222
Red=Cloning site Green=Tags(s)

MAASGESGASGGGGSTEEAFMTFYSEVKQIEKRDSVLTSKNQIERLTRPGSSYFNLNPFEVLQIDPEVTD
EEIKKRFRQLSILVHPDKNQDDADRAQKAFEAVDKAYKLLLDQEQKKRALDVIQAGKEYVEHTVKERKKQ
LKKEGKPTNVEEDDPELFKQAVYKQTMKLFAELEIKRKEREAKEMHERKRQREEEIEAQEKAKREREWQK
NFEESRDGRVDSWRNFQANTKGKKEKKNRTFLRPPKVKMEQRE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC066222
ORF Size 759 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC066222.1
RefSeq Size 1389 bp
RefSeq ORF 761 bp
Locus ID 68598
Cytogenetics 4 D2.3
MW 50.9 kDa
Summary Suppresses polyglutamine (polyQ) aggregation of ATXN3 in neuronal cells.UniProtKB/Swiss-Prot Function
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"BC066222" in other vectors (4)
SKU Description Size Price
MC218402 Dnajc8 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 8 (cDNA clone MGC:76784 IMAGE:30459247), (10ug) 10 ug
$330.00
MG203227 Dnajc8 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 8 (cDNA clone MGC:76784 IMAGE:30459247) 10 ug
$500.00
MR203227L3 Lenti ORF clone of Dnajc8 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 8 (cDNA clone MGC:76784 IMAGE:30459247) 10 ug
$600.00
MR203227L4 Lenti ORF clone of Dnajc8 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 8 (cDNA clone MGC:76784 IMAGE:30459247) 10 ug
$600.00

Plasmid Map for MR203227

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203227
Circular map for MR203227

file-alt Citations

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