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Ano9 (NM_178381) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203145
Ano9 (Myc-DDK-tagged) - Mouse anoctamin 9 (Ano9)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $684.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ano9
Synonyms 5430425C04Rik; Tmem16j; Tp53i5; Trp53i5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203145 representing NM_178381
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGATGATGAGAGTTCCCAGATCTTTATGGGGCCAGAAGGGGACCAGTTGCCATTGGTGGAAATGG
GCAGTTGTAAGCCTGAGGCCTCTGATCAATGGGACTGTGTCCTGGTGGCTGACCTCCAGACCCTGAAAAT
CCAGAAGCATGCTCAGAAGCAACTGCAGTTCCTAGAAAACCTCGAGAGCAATGGCTTCCACTTCAAGATG
TTAAAGGACCAGAAGAAGGTGTTCTTTGGGATCCGAGCTGACAGTGACGTCATTGACAAGTACCGGACTC
TCCTCATGAATCCTGAAGATTCTGGCTCCAGAGACGAGCAGAGCTTCAACATAGCTACCACAAGAATCCG
AATTGTGAGCTTTGTCGTGAACAACAAGCTAAAACCTGGTGACACCTTTGAGGACCTGGTGAAGGATGGG
GTTTTTGAGACCATGTTTCTTCTGCACAAGGGAGAACAAAACCTGAAGAATATATGGGCTCGGTGGAGAA
ACATGTTTGAACCACAGCCAATTGATGAAATCAGGGAATACTTTGGCGAGAAGGTGGCTCTGTACTTCAC
CTGGCTTGGCTGGTACACCTACATGCTGGTGCCTGCGGCTGTGGTGGGCCTCATCGTCTTTCTGAGTGGC
TTTGCCCTGTTTGATTCCAGCCAGATCAGCAAAGAGATCTGTAGTGCCAATGATATCTTCATGTGCCCTC
TTGGCGACCACAGTCACAGATACCTGCGACTCTCAGAGATGTGCACTTTCGCAAAGCTCACTCATCTCTT
TGACAACGAGGGCACCGTGCTATTTGCCATCTTCATGGCACTGTGGGCTACGGTCTTCCTGGAGATCTGG
AAGCGAAAGCGTGCCCATGAAGTCCAGAGCTGGAAACTGTATGAATGGGATGAGGAAGAGGAAGAGATGG
CCCTTGAGCTCATTAACTCTCCGCACTATAAGCTGAAAGACCACCGTCACTCCTACCTGAGCAGCACCAT
CATCCTGATCCTGTCCCTGTTCATGATCTGTTTAATGATTGGCATGGCTCATGTGCTGGTTGTCTACCGT
GTGCTGGCTGGAGCCCTCTTCAGCAGCCTCGTGAAGCAGCAGGTGACCACCGCTGTGGTGGTGACTGGGG
CAGTGGTACATTATATAATCATTGTCATCATGACTAAGGTCAACAAATATGTAGCCCTGAAGCTTTGTAA
ATTTGAGGAATCCGGGACCTTCTCAGAGCAAGAGAGGAAGTTCACTGTCAAGTTTTTCATCCTGCAGTTC
TTCGCCCACTTCTCCTCTCTCATCTACATTGCCTTCATTCTGGGCAGGATCAATGGTCACCCTGGGAAAT
CCACGCGCCTGGCTGGCCTGTGGAAGCTGGAGGAGTGCCATCTCAGTGGCTGCATGATGGACCTGTTTAT
CCAGATGGCCATCATCATGGGCCTGAAGCAAACCCTGAGCAACTGCGTTGAGTACCTGTGCCCGTTGTTG
GCCCATAAATGGCGCTTAATGTGGGCTTCCAAACATGGTCACATGTCCAAAGACCCAGAACTGAAGGAGT
GGCAGCGTAACTACTACATGAATCCCATCAACACCTTCAGCCTGTTTGATGAGTTCATGGAGATGATGAT
CCAGTACGGCTTTACCACCATCTTTGTGGCTGCTTTCCCACTCGCACCCCTGTTGGCGCTCTTCAGCAAC
CTTGTGGAAATCCGTCTGGATGCCATCAAGATGGTCAGACTACAGCGGCGCCTGGTTCCTCGCAAGGCTA
AGGACATAGGGACCTGGCTGCAGGTGCTAGAGACCATTGGAGTGCTGGCAGTCATTGCCAATGGAATGGT
TATCGCCTTCACCTCTGAGTTCATCCCCAGGGTAGTCTACAAGTACCACTATGGCCCGTGCCGAACGAAT
CGGACCTTCACAGATGACTGCCTCACGAACTATGTCAACCATAGCTTGTCTGTCTTCTACACCAAACACT
TCAATGACCACAGCAGGATGGAAGGCCAAGAAAACGTGACCGTGTGCAGGTACCGGGACTACCGAAATGA
GCATGACTACAACTTGTCTGAGCAGTTCTGGTTCATTTTGGCCATCCGACTGACCTTTGTTATCCTCTTT
GAGCATTTTGCCCTGTGCATCAAACTCATTGCTGCGTGGTTTGTGCCTGATGTGCCCCAGAAAGTGAAGA
ATGAGGTTCTGCAGGAGAAGTATGACAGAATTCGCCATAGAATGCGCTTCAGTTCCAGGAGCACGGATGT
A

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178381
ORF Size 2241 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178381.3, NP_848468.2
RefSeq Size 3012 bp
RefSeq ORF 2244 bp
Locus ID 71345
UniProt ID P86044
Cytogenetics 7 F5
MW 87.6 kDa
Summary Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (PubMed:23532839). Does not exhibit calcium-activated chloride channel (CaCC) activity (By similarity). Can inhibit the activity of ANO1 (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_178381" in other vectors (4)
SKU Description Size Price
MC221305 Ano9 (untagged) - Mouse anoctamin 9 (Ano9), (10ug) 10 ug
$753.00
MG203145 Ano9 (tGFP-tagged) - Mouse transformation related protein 53 inducible protein 5 (Trp53i5) 10 ug
$884.00
MR203145L3 Lenti ORF clone of Ano9 (Myc-DDK-tagged) - Mouse anoctamin 9 (Ano9) 10 ug
$984.00
MR203145L4 Lenti ORF clone of Ano9 (mGFP-tagged) - Mouse anoctamin 9 (Ano9) 10 ug
$984.00

Plasmid Map for MR203145

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203145
Circular map for MR203145

file-alt Citations

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