Tlcd1 (NM_026708) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203119
Tlcd1 (Myc-DDK-tagged) - Mouse TLC domain containing 1 (Tlcd1)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tlcd1
Synonyms 0610007A15Rik; 0610030G03Rik; AA690150; AU015616; RP23-185A18.3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203119 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCCTACTGTTCCACCCCGCCTGGCCGCTGCTCCTGGGCGCCACGCTGACCTTCCGGGCACTCCGGC
GCGTGCTCTGTCGCCTGCCCCAGCCTGCACACGTGCAAACCGACCCCCTGCGCACCTGGCGTTGGCACAA
CCTACTCGTCTCTTTCACCCACTCCATTGTATCAGGGATCTGGGCACTGCTGTGCTTATGGCAAACCCCT
GAAATGCTGGTGGAGATTGAAACAGCGTGGTCAGCTTCTGGCTATTTGCTTGTTTGCTTCTCTGCAGGAT
ACTTTATCCACGACACAGTGGACATTGTGGTTAGCAAGCAAACTCGAGCTTCTTGGGAATACCTTGTTCA
TCATGTCATGGCTATGGGCGCCTTCTTCTCTGGTATCTTTTGGAAAAGATTTGTTGGTGGTGGCGTCTTA
ACACTACTGGTAGAGGTCAGCAACATCTTCCTCACCTTAAGGATGATGATGAAGATAAACAATGCTCAGG
ATCTCCTCCTCTACAAGGTCAACAAGTATATCAACTTGGTCATGTACTTCCTCTTCCGATTGGCCCCTCA
GGCCTACCTCACCAAGTTCTTCCTACAGTATGCTGGCCAGAGGACCCTGGGCACATTCTTGTTGGCCATC
CTGCTCATGCTGGACTTGATGATAATCATCTACTTTTCTCGCCTCCTCCGCTCTGATTTCTGCCCTGAAC
GTGCACCCAGGCGCCAACAAAAAGACAAATTCTTGACTGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203119 protein sequence
Red=Cloning site Green=Tags(s)

MPLLFHPAWPLLLGATLTFRALRRVLCRLPQPAHVQTDPLRTWRWHNLLVSFTHSIVSGIWALLCLWQTP
EMLVEIETAWSASGYLLVCFSAGYFIHDTVDIVVSKQTRASWEYLVHHVMAMGAFFSGIFWKRFVGGGVL
TLLVEVSNIFLTLRMMMKINNAQDLLLYKVNKYINLVMYFLFRLAPQAYLTKFFLQYAGQRTLGTFLLAI
LLMLDLMIIIYFSRLLRSDFCPERAPRRQQKDKFLTE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026708
ORF Size 741 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026708.2
RefSeq Size 1454 bp
RefSeq ORF 744 bp
Locus ID 68385
UniProt ID Q99JT6
Cytogenetics 11 B5
MW 28.8 kDa
Summary Regulates the composition and fluidity of the plasma membrane (By similarity). Inhibits the incorporation of membrane-fluidizing phospholipids containing omega-3 long-chain polyunsaturated fatty acids (LCPUFA) and thereby promotes membrane rigidity (By similarity). Does not appear to have any effect on LCPUFA synthesis (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026708" in other vectors (4)
SKU Description Size Price
MC200546 Tlcd1 (untagged) - Mouse TLC domain containing 1 (Tlcd1), (10ug) 10 ug
$300.00
MG203119 Tlcd1 (tGFP-tagged) - Mouse TLC domain containing 1 (Tlcd1) 10 ug
$500.00
MR203119L3 Lenti ORF clone of Tlcd1 (Myc-DDK-tagged) - Mouse TLC domain containing 1 (Tlcd1) 10 ug
$600.00
MR203119L4 Lenti ORF clone of Tlcd1 (mGFP-tagged) - Mouse TLC domain containing 1 (Tlcd1) 10 ug
$600.00

Plasmid Map for MR203119

Download sequence for

Cloning scheme for MR203119

Circular map for MR203119

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