Prss1 (NM_053243) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203088
Prss1 (Myc-DDK-tagged) - Mouse protease, serine, 1 (trypsin 1) (Prss1)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Prss1
Synonyms Try-1; Try1; Trygn16
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203088 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGCACTCCTGTTCCTGGCCCTTGTGGGAGCTGCTGTTGCTTTCCCTGTGGATGATGATGACAAGA
TCGTTGGAGGATACACCTGCCGAGAGAATTCTGTTCCCTACCAGGTGTCCCTGAACTCTGGCTACCACTT
CTGTGGAGGTTCCCTCATCAATGACCAGTGGGTGGTGTCTGCAGCTCATTGCTACAAGACCCGCATCCAA
GTGAGATTGGGGGAGCACAATATCAATGTCCTGGAGGGCAATGAGCAGTTTATCGATGCTGCCAAGATCA
TCAAGCACCCCAACTTCAATAGGAAGACCCTGAACAATGACATCATGTTGATCAAGCTCTCATCCCCTGT
GACCCTCAATGCCAGAGTGGCCACTGTGGCTCTGCCCAGCTCCTGTGCACCTGCTGGCACTCAGTGTCTC
ATCTCTGGCTGGGGCAACACTCTTAGCTTTGGTGTCAGTGAACCAGACCTGCTCCAGTGCCTGGATGCCC
CACTGCTGCCTCAGGCTGACTGTGAGGCTTCCTATCCTGGGAAGATCACTGGTAACATGGTCTGTGCTGG
CTTCCTAGAGGGAGGCAAGGATTCCTGCCAGGGTGACTCTGGTGGCCCTGTGGTCTGCAATGGAGAGCTC
CAGGGCATTGTCTCTTGGGGCTATGGCTGTGCCCTGCCAGACAATCCTGGTGTGTACACCAAGGTCTGCA
ACTATGTGGATTGGATTCAGGACACAATTGCTGCCAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203088 protein sequence
Red=Cloning site Green=Tags(s)

MSALLFLALVGAAVAFPVDDDDKIVGGYTCRENSVPYQVSLNSGYHFCGGSLINDQWVVSAAHCYKTRIQ
VRLGEHNINVLEGNEQFIDAAKIIKHPNFNRKTLNNDIMLIKLSSPVTLNARVATVALPSSCAPAGTQCL
ISGWGNTLSFGVSEPDLLQCLDAPLLPQADCEASYPGKITGNMVCAGFLEGGKDSCQGDSGGPVVCNGEL
QGIVSWGYGCALPDNPGVYTKVCNYVDWIQDTIAAN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_053243
ORF Size 738 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_053243.2, NP_444473.1
RefSeq Size 807 bp
RefSeq ORF 741 bp
Locus ID 114228
UniProt ID Q9Z1R9
Cytogenetics 6 19.6 cM
MW 26.1 kDa
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Documents
Resources
"NM_053243" in other vectors (4)
SKU Description Size Price
MC207841 Prss1 (untagged) - Mouse protease, serine, 1 (trypsin 1) (Prss1), (10ug) 10 ug
$330.00
MG203088 Prss1 (tGFP-tagged) - Mouse protease, serine, 1 (trypsin 1) (Prss1) 10 ug
$500.00
MR203088L3 Lenti ORF clone of Prss1 (Myc-DDK-tagged) - Mouse protease, serine, 1 (trypsin 1) (Prss1) 10 ug
$600.00
MR203088L4 Lenti ORF clone of Prss1 (mGFP-tagged) - Mouse protease, serine, 1 (trypsin 1) (Prss1) 10 ug
$600.00

Plasmid Map for MR203088

Download sequence for

Cloning scheme for MR203088

Circular map for MR203088

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