Trp53rkb (NM_023815) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203051
Trp53rk (Myc-DDK-tagged) - Mouse transformation related protein 53 regulating kinase (Trp53rk)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Trp53rkb
Synonyms 2810408M09Rik; 4933401B08Rik; 5630401H01Rik; mNori-2p; Nori-2; PRPK; Tp53rk; Trp53rk
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203051 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGGTGTGTCCTCGGAGGCGGAGGCCGAGGCGCTGGCCGCGGCGCGCGAGCGGAGCCGCCTCTTCC
TGAGCGGCCTAGAGCTGGTGCAGCAGGGCGCCGAGGCGCGCGTTTTCCGTGGCCGCTTCCAGGGCCGCGC
GGCCGTGGTGAAGCACCGCTTCCCGAAGAGTTACCGGCACCCGGAGCTGGAGGCGCGGCTCGGCCGTCGG
CGGACGGTGCAGGAGGCGCGCGCGCTGCTCCGCTGCCGCCGTGCGGGGATAGCTGCCCCAGTCGTCTTCT
TTGTGGACTATGCGTCTAACTGCTTATATATGGAAGAAATCGAAGACTCGGTGACTGTTCGGGATTATAT
CCAATCCACTATGGAGACTGAAAAGGACCCCCAGTGCCTCTTGGACCTGGCCAGGAGGATGGGGCAGGTT
CTGGCCGGAATGCACGACCAAGACCTCATTCACGGGGACCTCACCACCTCCAACATGCTCCTGAGGCGGC
CCCTGGCGCAGCTGCACATCGTGCTCATCGACTTTGGGCTGAGCTTTGTCTCAGGACTGCCGGAAGATAA
AGGCGTCGACCTCTATGTCCTGGAGAAGGCCTTCCTCAGCACGCACCCCCACACCGAGACCGCGTTTGAA
GCCTTTCTGAAGAGTTACGGGGCCTCGTCCAAGAAGTCCAGTCCAGTGCTGAAGAAGTTAGATGAGGTGC
GCCTGAGAGGGCGAAAGCGGTCCATGGTCGGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203051 protein sequence
Red=Cloning site Green=Tags(s)

MAGVSSEAEAEALAAARERSRLFLSGLELVQQGAEARVFRGRFQGRAAVVKHRFPKSYRHPELEARLGRR
RTVQEARALLRCRRAGIAAPVVFFVDYASNCLYMEEIEDSVTVRDYIQSTMETEKDPQCLLDLARRMGQV
LAGMHDQDLIHGDLTTSNMLLRRPLAQLHIVLIDFGLSFVSGLPEDKGVDLYVLEKAFLSTHPHTETAFE
AFLKSYGASSKKSSPVLKKLDEVRLRGRKRSMVG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023815
ORF Size 732 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023815.4, NP_076304.2
RefSeq Size 4413 bp
RefSeq ORF 735 bp
Locus ID 76367
Cytogenetics 2 H3
MW 27.4 kDa
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Documents
Resources
"NM_023815" in other vectors (3)
SKU Description Size Price
MC207760 Trp53rk (untagged) - Mouse transformation related protein 53 regulating kinase (Trp53rk), (10ug) 10 ug
$300.00
MR203051L3 Lenti ORF clone of Trp53rk (Myc-DDK-tagged) - Mouse transformation related protein 53 regulating kinase (Trp53rk) 10 ug
$600.00
MR203051L4 Lenti ORF clone of Trp53rk (mGFP-tagged) - Mouse transformation related protein 53 regulating kinase (Trp53rk) 10 ug
$600.00

Plasmid Map for MR203051

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Cloning scheme for MR203051

Circular map for MR203051

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