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Vapb (NM_019806) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203021
Vapb (Myc-DDK-tagged) - Mouse vesicle-associated membrane protein, associated protein B and C (Vapb)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vapb
Synonyms AI225786; AI840687; AI848259; D2Abb2e; R75548; Vamp33b; VAP33b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203021 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAAGGTGGAACAGGTCCTGAGCCTCGAGCCACAACACGAGCTCAAGTTCCGAGGTCCCTTCACTG
ATGTTGTCACCACCAACCTAAAGCTTGGCAACCCAACAGACCGAAATGTGTGTTTTAAAGTGAAGACCAC
AGCACCTCGCAGGTACTGCGTGCGGCCCAACAGTGGGGTCATTGATGCCGGGGCCTCTCTCAATGTGTCT
GTGATGTTACAGCCTTTCGATTATGATCCCAATGAGAAAAGTAAACACAAGTTTATGGTTCAGTCTATGT
TTGCTCCGCCTGACACTTCTGATATGGAGGCAGTATGGAAGGAGGCAAAACCGGAAGACCTTATGGATTC
AAAACTTAGATGTGTGTTTGAATTGCCAGCAGAAAATGCTAAACCACATGATGTAGAAATAAATAAAATC
ATACCTACGAGTGCATCCAAGACAGAAGCACCGGCAGCCGCAAAGTCCCTGACATCGCCCCTCGATGACA
CAGAAGTAAAGAAGGTGATGGAAGAGTGCAGGCGGCTGCAGGGGGAGGTGCAGAGGCTTCGGGAGGAGAG
CAGGCAGCTCAAGGAAGAAGACGGACTTCGGGTGAGGAAGGCGATGCCGAGCAACAGCCCCGTGGCGGCT
CTGGCGGCCACTGGGAAGGAGGAGGGCCTGAGCGCCCGGCTGCTGGCCCTGGTGGTTCTGTTCTTTATCG
TTGGTGTCATTATAGGGAAGATTGCCTTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203021 protein sequence
Red=Cloning site Green=Tags(s)

MAKVEQVLSLEPQHELKFRGPFTDVVTTNLKLGNPTDRNVCFKVKTTAPRRYCVRPNSGVIDAGASLNVS
VMLQPFDYDPNEKSKHKFMVQSMFAPPDTSDMEAVWKEAKPEDLMDSKLRCVFELPAENAKPHDVEINKI
IPTSASKTEAPAAAKSLTSPLDDTEVKKVMEECRRLQGEVQRLREESRQLKEEDGLRVRKAMPSNSPVAA
LAATGKEEGLSARLLALVVLFFIVGVIIGKIAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019806
ORF Size 729 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019806.5, NP_062780.2
RefSeq Size 6969 bp
RefSeq ORF 732 bp
Locus ID 56491
Cytogenetics 2 97.37 cM
MW 26.9 kDa
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Documents
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"NM_019806" in other vectors (4)
SKU Description Size Price
MC203260 Vapb (untagged) - Mouse vesicle-associated membrane protein, associated protein B and C (Vapb), (10ug) 10 ug
$300.00
MG203021 Vapb (tGFP-tagged) - Mouse vesicle-associated membrane protein, associated protein B and C (Vapb) 10 ug
$500.00
MR203021L3 Lenti ORF clone of Vapb (Myc-DDK-tagged) - Mouse vesicle-associated membrane protein, associated protein B and C (Vapb) 10 ug
$600.00
MR203021L4 Lenti ORF clone of Vapb (mGFP-tagged) - Mouse vesicle-associated membrane protein, associated protein B and C (Vapb) 10 ug
$600.00

Plasmid Map for MR203021

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203021
Circular map for MR203021

file-alt Citations

Identification of VAPA and VAPB as Kv2 channel-interacting proteins defining endoplasmic reticulum-plasma membrane junctions in mammalian brain neurons
Kirmiz, Vierra, Palacio et al
J. Neurosci. (2018)
AI Summary: OriGene’s mouse VAPB plasmid (Cat# MR203021) was used to generate a GFP-tagged VAPB construct for expression studies, enabling the investigation of VAPB’s interaction with Kv2 channels in neuronal ER-PM junctions.
Snippet: The plasmid encoding GFP-VAPB 310 was generated by amplifying mouse VAPB (Origene Cat# MR203021) by PCR using primers to 311 introduce a KpnI site upstream of the start codon, and an AgeI site on the 3' end of VAPB. The 312
Showing 1-1 of 1 papers.
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