OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Tfpi2 (NM_009364) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR202782
Tfpi2 (Myc-DDK-tagged) - Mouse tissue factor pathway inhibitor 2 (Tfpi2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Tfpi2 or NM_009364"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tfpi2
Synonyms AV000670; PP5/TFPI-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202782 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCCTGCTATGCCCCTGCAACTGTGGAATTTGCCGCTGCTCCTGGTGGGCTCCGTTCTTGGTCTCA
CTTCAGTGTCTGCCCAAGGGAATAATTTAGAGATCTGCCTCTTGCCCTTGGACGCAGGACCTTGTCAGGC
CCTCATCCCCAAGTTCTACTATGACAGAGACCAGCAAAAGTGCCGCAGATTCAACTATGGAGGCTGCTTG
GGCAATGCCAACAATTTCCACAGTCGGGACCTCTGCCAACAGACTTGCGGAAGTATCGAGAAAGTTCCTC
CAGTTTGTCGATCAGAGCTAAAAACATATCCATGTGATAAGCCCAATATACGGTTTTTCTTCAACCTGAA
TACCATGACATGTGAGCCCCTTAGGCCTGGTCTGTGTAGCAGGACTATAAATGTATTCTCTGAGGAAGCT
ACATGTAAGGGCTTGTGTGAACCACGGAAACATATTCCATCATTTTGCTCCAGTCCAAAGGATGAAGGTC
TGTGTTCTGCCAATGTGACACGCTTTTATTTTAATTCAAGAAACAAAACATGTGAGACTTTCACCTATAC
TGGCTGTGGTGGGAATGAGAATAACTTTTATTACCTGGATGCTTGCCACCGTGCCTGCGTTAAAGGCTGG
AAGAAGCCCAAGAGATGGAAGATAGGTGACTTCCTGCCCAGATTTTGGAAGCATCTTTCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR202782 protein sequence
Red=Cloning site Green=Tags(s)

MDPAMPLQLWNLPLLLVGSVLGLTSVSAQGNNLEICLLPLDAGPCQALIPKFYYDRDQQKCRRFNYGGCL
GNANNFHSRDLCQQTCGSIEKVPPVCRSELKTYPCDKPNIRFFFNLNTMTCEPLRPGLCSRTINVFSEEA
TCKGLCEPRKHIPSFCSSPKDEGLCSANVTRFYFNSRNKTCETFTYTGCGGNENNFYYLDACHRACVKGW
KKPKRWKIGDFLPRFWKHLS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009364
ORF Size 690 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009364.4
RefSeq Size 1522 bp
RefSeq ORF 693 bp
Locus ID 21789
UniProt ID O35536
Cytogenetics 6 1.81 cM
MW 26.1 kDa
Summary May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_009364" in other vectors (4)
SKU Description Size Price
MC203764 Tfpi2 (untagged) - Mouse tissue factor pathway inhibitor 2 (Tfpi2), (10ug) 10 ug
$300.00
MG202782 Tfpi2 (tGFP-tagged) - Mouse tissue factor pathway inhibitor 2 (Tfpi2) 10 ug
$500.00
MR202782L3 Lenti ORF clone of Tfpi2 (Myc-DDK-tagged) - Mouse tissue factor pathway inhibitor 2 (Tfpi2) 10 ug
$600.00
MR202782L4 Lenti ORF clone of Tfpi2 (mGFP-tagged) - Mouse tissue factor pathway inhibitor 2 (Tfpi2) 10 ug
$600.00

Plasmid Map for MR202782

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR202782
Circular map for MR202782

file-alt Citations

Restoration of TFPI2 by LSD1 inhibition suppresses tumor progression and potentiates antitumor immunity in breast cancer
Gu, Vasilatos, Yin et al
Cancer Lett (2024) 600, 217182 DOI: 10.1016/j.canlet.2024.217182
Cited by 13
AI Summary: OriGene products, specifically the plasmids pCMV6-Entry, pCMV6-Entry-Tfpi2 (mouse), pCMV6-Entry-Cxcl9 (mouse), and pCMV6-Entry-Cxcl10 (mouse), were used in this study to manipulate gene expression and investigate the roles of TFPI2 and chemokines in breast cancer progression and immune response.
Snippet: Plasmids pCMV6-Entry, pCMV6-Entry-Tfpi2 (mouse), pCMV6-Entry-Cxcl9 (mouse) and pCMV6-Entry-Cxcl10 (mouse) were purchased from OriGene Technologies, Inc (Rockville, MD). MDA-MB-231, 4T1, EMT6, T47D and SKBR3 cell lines were obtained from ATCC (Manassas, VA). Cells were cultured in growth medium as described previously [ 10 , 17 , 18 ]
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products