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Gjb2 (NM_008125) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR202661
Gjb2 (Myc-DDK-tagged) - Mouse gap junction protein, beta 2 (Gjb2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$289.00 $450.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Gjb2 or NM_008125"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gjb2
Synonyms AI325222; Cnx26; Cx26; Gjb-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202661 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATTGGGGCACACTCCAGAGCATCCTCGGGGGTGTCAACAAACACTCCACCAGCATTGGAAAGATCT
GGCTCACGGTCCTCTTCATCTTCCGCATCATGATCCTCGTGGTGGCTGCAAAGGAGGTGTGGGGAGATGA
GCAAGCCGATTTTGTCTGCAACACGCTCCAGCCTGGCTGCAAGAATGTATGCTACGACCACCACTTCCCC
ATCTCTCACATCCGGCTCTGGGCTCTGCAGCTGATCATGGTGTCCACGCCAGCCCTCCTGGTAGCTATGC
ATGTGGCCTACCGGAGACATGAAAAGAAACGGAAGTTCATGAAGGGAGAGATAAAGAACGAGTTTAAGGA
CATCGAAGAGATCAAAACCCAGAAGGTCCGTATCGAAGGGTCCCTGTGGTGGACCTACACCACCAGCATC
TTCTTCCGGGTCATCTTTGAAGCCGTCTTCATGTACGTCTTTTACATCATGTACAATGGCTTCTTCATGC
AACGTCTGGTGAAATGCAACGCTTGGCCCTGCCCCAATACAGTGGACTGCTTCATTTCCAGGCCCACAGA
AAAGACTGTCTTCACCGTGTTTATGATTTCTGTGTCTGGAATTTGCATTCTGCTAAATATCACAGAGCTG
TGCTATTTGTTCGTTAGGTATTGCTCAGGAAAGTCCAAAAGACCAGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR202661 protein sequence
Red=Cloning site Green=Tags(s)

MDWGTLQSILGGVNKHSTSIGKIWLTVLFIFRIMILVVAAKEVWGDEQADFVCNTLQPGCKNVCYDHHFP
ISHIRLWALQLIMVSTPALLVAMHVAYRRHEKKRKFMKGEIKNEFKDIEEIKTQKVRIEGSLWWTYTTSI
FFRVIFEAVFMYVFYIMYNGFFMQRLVKCNAWPCPNTVDCFISRPTEKTVFTVFMISVSGICILLNITEL
CYLFVRYCSGKSKRPV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008125
ORF Size 678 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008125.3
RefSeq Size 2404 bp
RefSeq ORF 681 bp
Locus ID 14619
UniProt ID Q00977
Cytogenetics 14 30.1 cM
MW 26.4 kDa
Summary Structural component of gap junctions (PubMed:15692151). Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane (By similarity). Small molecules and ions diffuse from one cell to a neighboring cell via the central pore (PubMed:15692151).UniProtKB/Swiss-Prot Function
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"NM_008125" in other vectors (4)
SKU Description Size Price
MC201034 Gjb2 (untagged) - Mouse gap junction protein, beta 2 (Gjb2), (10ug) 10 ug
$450.00
MG202661 Gjb2 (tGFP-tagged) - Mouse gap junction membrane channel protein beta 2 (Gjb2) 10 ug
$489.00 $650.00
MR202661L3 Lenti ORF clone of Gjb2 (Myc-DDK-tagged) - Mouse gap junction protein, beta 2 (Gjb2) 10 ug
$750.00
MR202661L4 Lenti ORF clone of Gjb2 (mGFP-tagged) - Mouse gap junction protein, beta 2 (Gjb2) 10 ug
$750.00

Plasmid Map for MR202661

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR202661
Circular map for MR202661

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