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Atp6v1e2 (NM_029121) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR202655
Atp6v1e2 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit E2 (Atp6v1e2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atp6v1e2
Synonyms 4930500C14Rik; Atp6e1; E1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202655 representing NM_029121
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTGACTGACATAGACGTGCAGAAGCAGATCAAGCACATGATGGCGTTCATCGAGCAGGAAGCCA
ACGAGAAGGCGGAGGAGATAGACGCCAAGGCGGAGGAAGAGTTCAACATTGAGAAGGGCCGCCTGGTGCA
GACCCAGCGGCTGAAGATCATGGATTATTTTGAGAAGAAGGAGAAACAGATTGAGCAGCAGAAAAAGATC
CAGCTGTCCACCATGAGAAACCAGGCTCGCATCACGGTCCTGAGAGCCCGAGACAATCTCATCTTAGAAT
TGCTCAAGGATGCCAAGATGAGGCTCAGCAGAATTGTGTCGGATGAAGAAATCTACCAGGACCTGCTGGA
TAAGCTCGTGCTCCAAGCCTTGCTCCGGCTGCTGGAGCCTGTGATGATTGTCCGCTGCCGGCCACAGGAC
CTCCACCTGGTGGAGTCTGCGGTGCTAAGAGCCATCCCTCAGTACATGAGGCTCTGCCAGAAGCATTTGG
AGGTACAGGTTGACCAAACTGAACACTTGCCTTCCAATGCTGCTGGAGGGGTGGAGGTCTACAGCAGCGA
TCAGAAAATAAAGGTTTCCAACACCCTGGAAAGTCGGCTGAATCTCGCGGCCATGCAGAAGATGCCAGAA
ATACGAGGGATTCTGTTTGGAGACAACACCAGCAGAAAGTTCTTTACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR202655 representing NM_029121
Red=Cloning site Green=Tags(s)

MALTDIDVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMDYFEKKEKQIEQQKKI
QLSTMRNQARITVLRARDNLILELLKDAKMRLSRIVSDEEIYQDLLDKLVLQALLRLLEPVMIVRCRPQD
LHLVESAVLRAIPQYMRLCQKHLEVQVDQTEHLPSNAAGGVEVYSSDQKIKVSNTLESRLNLAAMQKMPE
IRGILFGDNTSRKFFT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029121
ORF Size 678 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029121.3, NP_083397.3
RefSeq Size 1005 bp
RefSeq ORF 681 bp
Locus ID 74915
UniProt ID Q9D593
Cytogenetics 17 E4
MW 26.3 kDa
Summary Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. This isoform is essential for energy coupling involved in acidification of acrosome.UniProtKB/Swiss-Prot Function
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"NM_029121" in other vectors (4)
SKU Description Size Price
MC204961 Atp6v1e2 (untagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit E2 (Atp6v1e2), (10ug) 10 ug
$300.00
MG202655 Atp6v1e2 (tGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit E2 (Atp6v1e2) 10 ug
$500.00
MR202655L3 Lenti ORF clone of Atp6v1e2 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit E2 (Atp6v1e2) 10 ug
$600.00
MR202655L4 Lenti ORF clone of Atp6v1e2 (mGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit E2 (Atp6v1e2) 10 ug
$600.00

Plasmid Map for MR202655

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR202655
Circular map for MR202655

file-alt Citations

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