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Scn1b (NM_011322) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR202467
Scn1b (Myc-DDK-tagged) - Mouse sodium channel, voltage-gated, type I, beta (Scn1b)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Scn1b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202467 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGACGCTGCTGGCTCTCGTGGTGGGCGCGGCGCTGGTATCCTCAGCCTGGGGGGGCTGCGTGGAGG
TGGATTCCGATACCGAGGCTGTGTATGGGATGACCTTCAAAATCCTGTGCATCTCCTGTAAGCGTCGTAG
TGAGACCACCGCCGAAACCTTCACGGAGTGGACCTTCCGCCAGAAGGGGACAGAGGAATTTGTCAAGATC
CTACGCTATGAGAATGAGGTGCTGCAGCTGGAGGAAGATGAGCGCTTTGAGGGCCGAGTGGTGTGGAACG
GTAGTCGGGGCACCAAGGACCTGCAGGACCTGTCCATCTTCATCACCAACGTCACCTACAACCACTCTGG
CGACTACGAATGTCACGTCTACCGTCTCCTCTTCTTTGATAATTATGAGCACAACACCAGCGTCGTCAAG
AAGATCCACCTGGAGGTGGTGGACAAGGCCAACAGAGATATGGCATCCATCGTGTCAGAGATCATGATGT
ACGTGCTCATTGTGGTGTTGACCATATGGCTTGTGGCAGAGATGGTGTACTGCTACAAGAAGATTGCTGC
TGCTACGGAGGCTGCTGCACAAGAGAACGCCTCAGAATACCTGGCCATTACATCCGAGAGCAAAGAAAAC
TGTACAGGCGTCCAGGTGGCTGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR202467 protein sequence
Red=Cloning site Green=Tags(s)

MGTLLALVVGAALVSSAWGGCVEVDSDTEAVYGMTFKILCISCKRRSETTAETFTEWTFRQKGTEEFVKI
LRYENEVLQLEEDERFEGRVVWNGSRGTKDLQDLSIFITNVTYNHSGDYECHVYRLLFFDNYEHNTSVVK
KIHLEVVDKANRDMASIVSEIMMYVLIVVLTIWLVAEMVYCYKKIAAATEAAAQENASEYLAITSESKEN
CTGVQVAE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011322
ORF Size 654 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011322.3
RefSeq Size 1568 bp
RefSeq ORF 657 bp
Locus ID 20266
UniProt ID P97952
Cytogenetics 7 19.3 cM
MW 24.7 kDa
Summary Regulatory subunit of multiple voltage-gated sodium channel complexes that play important roles in excitable membranes in brain, heart and skeletal muscle. Enhances the presence of the pore-forming alpha subunit at the cell surface and modulates channel gating characteristics and the rate of channel inactivation. Modulates the activity of a variety of pore-forming alpha subunits, such as SCN1A, SCN2A, SCN3A, SCN4A, SCN5A and SCN10A.UniProtKB/Swiss-Prot Function
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"NM_011322" in other vectors (4)
SKU Description Size Price
MC207389 Scn1b (untagged) - Mouse sodium channel, voltage-gated, type I, beta (Scn1b), (10ug) 10 ug
$330.00
MG202467 Scn1b (tGFP-tagged) - Mouse sodium channel, voltage-gated, type I, beta (Scn1b) 10 ug
$489.00 $500.00
MR202467L3 Lenti ORF clone of Scn1b (Myc-DDK-tagged) - Mouse sodium channel, voltage-gated, type I, beta (Scn1b) 10 ug
$600.00
MR202467L4 Lenti ORF clone of Scn1b (mGFP-tagged) - Mouse sodium channel, voltage-gated, type I, beta (Scn1b) 10 ug
$600.00

Plasmid Map for MR202467

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR202467
Circular map for MR202467

file-alt Citations

Sexually Divergent Mortality and Partial Phenotypic Rescue after Gene Therapy in a Mouse Model of Dravet Syndrome
Niibori, Lee, Minassian et al
Hum. Gene Ther. (2019) 31 (5-6), 339-351 DOI: 10.1089/hum.2019.225
Cited by 75
AI Summary: OriGene’s Scn1b DNA plasmid was used as the template to amplify the NaVβ1 gene by PCR, enabling the construction of the gene therapy vector tested in the study.
Snippet: 3') and 3' primer (5' - gcatGGATCCaAGATCTGTCCGGGTGATCCGGTATTT - 3'). The mouse Scn1b gene encoding NaVβ1 protein was amplified from an Scn1b encoding DNA plasmid (Origene) by PCR using Top Taq DNA polymerase (QIAGEN)
Showing 1-1 of 1 papers.
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