1700129C05Rik (NM_026461) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR202447
1700129C05Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700129C05 gene (1700129C05Rik)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 1700129C05Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202447 representing NM_026461
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGAGAAGGAAGCGGCCAAGGATTTTGGGAAGTCTACGCAATCCTGTTCCCCCTAAGGATCTTTCTC
TGCCCTCTGATCAACCAAAAAACATTCATTTCTTCCCACCTCAGACTCCCATGCCTCAGCCTACCTTTCG
TACCAACCCTCTTTTCGTTTTCTACCCTACCTCCTTCCCTGCCCTGAGGAACACTTCCTACTTCCCCACC
TTAATGATTGCTATGCCCAGCTCCTCCTGTGTAAACAAACTTTCCTCTAAGCACCCCTCTTATTTCCAGA
CCCCTTCCTTCCTGTCTACCCTCATGTCTACCTCTTCTCCAAGAGCACTGCTGGATAGTTGCCTAGTTCC
TCTTTCCCATCTGGAAACTAGCTACTTTCAGCCAGAGGAGCAAATCCTTCAGGAACTGAACAAGATTTCT
CTAGACCCAGAGCTTAAAATTGGAGGAAGCAGATATGACCTCACATCCTTGGTACCAGGGAATCTGCAGC
CAGGGACAGAGACTGGAAATCATGAAGTGGAAGATGCTCAATCCCTGGATGATCTTGATGCCTTCACTCA
GGAAACACTGGACAGAATGAATTGTCATTGGTCAAGAACGCAAGTTTTACCAAATAACATTTCGGGATGG
AGAAACCAGAGGGGGAAACTGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR202447 representing NM_026461
Red=Cloning site Green=Tags(s)

MMRRKRPRILGSLRNPVPPKDLSLPSDQPKNIHFFPPQTPMPQPTFRTNPLFVFYPTSFPALRNTSYFPT
LMIAMPSSSCVNKLSSKHPSYFQTPSFLSTLMSTSSPRALLDSCLVPLSHLETSYFQPEEQILQELNKIS
LDPELKIGGSRYDLTSLVPGNLQPGTETGNHEVEDAQSLDDLDAFTQETLDRMNCHWSRTQVLPNNISGW
RNQRGKLK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026461
ORF Size 654 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026461.2, NP_080737.1
RefSeq Size 1067 bp
RefSeq ORF 657 bp
Locus ID 67932
Cytogenetics 14 C3
MW 24.7 kDa
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Documents
Resources
"NM_026461" in other vectors (4)
SKU Description Size Price
MC204923 1700129C05Rik (untagged) - Mouse RIKEN cDNA 1700129C05 gene (1700129C05Rik), (10ug) 10 ug
$300.00
MG202447 1700129C05Rik (tGFP-tagged) - Mouse RIKEN cDNA 1700129C05 gene (1700129C05Rik) 10 ug
$500.00
MR202447L3 Lenti ORF clone of 1700129C05Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700129C05 gene (1700129C05Rik) 10 ug
$600.00
MR202447L4 Lenti ORF clone of 1700129C05Rik (mGFP-tagged) - Mouse RIKEN cDNA 1700129C05 gene (1700129C05Rik) 10 ug
$600.00

Plasmid Map for MR202447

Download sequence for

Cloning scheme for MR202447

Circular map for MR202447

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