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Nudt5 (NM_016918) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR202441
Nudt5 (Myc-DDK-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 5 (Nudt5)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nudt5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202441 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACCCGAGAATCCACAGAGTCTTCTCCAGGCAAGCACCTTGTTACCTCAGAGGAGTTGATCTCAG
AAGGAAAATGGGTCAAATTTGAAAAAACAACTTATATGGATCCCACTGGTAAAACCAGAACTTGGGAAAC
AGTGAAACTTACAACCAGGAAGGGAAAATCTGCTGATGCCGTGTCGGTCATACCTGTGCTGCAAAGAACC
CTGCACCATGAGTGCGTCATCCTGGTGAAGCAGTTCCGGCCCCCGATGGGCAGCTACTGCCTGGAGTTTC
CAGCAGGGTTCATCGAAGACGGAGAAAACCCAGAGGCGGCTGCTCTTCGGGAGCTGGAGGAAGAAACTGG
CTACAAAGGTGAAGTTGCGGAATGCTCTCCAGCTGTGTGCATGGATCCAGGCTTGTCAAACTGCACCACA
CATGTTGTGACAGTGACCATCAATGGAGATGATGCAGGAAATGTAAGGCCAAAACCCAAACCAGGGGATG
GAGAATTTATGGAAGTGATTTCTTTACCAAAGAATGATCTGCTGACAAGACTTGACGCTTTGGGAGCAGA
ACAACACCTTACAGTGGATGCCAAGGTCTACGCCTACGGTCTGGCTCTGAAACACGCCAACTCGAAGCCA
TTCGAAGTGCCCTTCCTCAAATTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR202441 protein sequence
Red=Cloning site Green=Tags(s)

METRESTESSPGKHLVTSEELISEGKWVKFEKTTYMDPTGKTRTWETVKLTTRKGKSADAVSVIPVLQRT
LHHECVILVKQFRPPMGSYCLEFPAGFIEDGENPEAAALRELEEETGYKGEVAECSPAVCMDPGLSNCTT
HVVTVTINGDDAGNVRPKPKPGDGEFMEVISLPKNDLLTRLDALGAEQHLTVDAKVYAYGLALKHANSKP
FEVPFLKF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016918
ORF Size 654 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016918.1, NM_016918.2, NM_016918.3, NP_058614.1
RefSeq Size 1590 bp
RefSeq ORF 657 bp
Locus ID 53893
UniProt ID Q9JKX6
Cytogenetics 2 A1
MW 24 kDa
Summary Enzyme that can either act as an ADP-sugar pyrophosphatase in absence of diphosphate or catalyze the synthesis of ATP in presence of diphosphate (By similarity). In absence of diphosphate, hydrolyzes with similar activities various modified nucleoside diphosphates such as ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP (PubMed:10722730). Can also hydrolyze other nucleotide sugars with low activity (PubMed:10722730). In presence of diphosphate, mediates the synthesis of ATP in the nucleus by catalyzing the conversion of ADP-ribose to ATP and ribose 5-phosphate (By similarity). Nuclear ATP synthesis takes place when dephosphorylated at Thr-44 (By similarity). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (By similarity). Does not play a role in U8 snoRNA decapping activity (PubMed:21070968). Binds U8 snoRNA (PubMed:21070968).UniProtKB/Swiss-Prot Function
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"NM_016918" in other vectors (4)
SKU Description Size Price
MC204897 Nudt5 (untagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 5 (Nudt5), (10ug) 10 ug
$300.00
MG202441 Nudt5 (tGFP-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 5 (Nudt5) 10 ug
$500.00
MR202441L3 Lenti ORF clone of Nudt5 (Myc-DDK-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 5 (Nudt5) 10 ug
$600.00
MR202441L4 Lenti ORF clone of Nudt5 (mGFP-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 5 (Nudt5) 10 ug
$600.00

Plasmid Map for MR202441

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR202441
Circular map for MR202441

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