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Alkbh7 (BC029677) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR202335
Alkbh7 (Myc-DDK-tagged) - Mouse alkB, alkylation repair homolog 7 (E. coli) (cDNA clone MGC:39017 IMAGE:5364485)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
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Other products for "Alkbh7 or BC029677"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Alkbh7
Synonyms 2310045B01Rik; 2510008E23Rik; Abh7; Spata11
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202335 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGTTGCTTTCTGGGTGCGCCTGGGTGCGCGGCTCAGACTCTGCCGTGCTGGGCCGCCTGCGTGATG
AGGCCGTGGTGCATCCAGGCTTCCTGAGCCAGGAGGAGGAGGACACGCTAACACGCGAACTGGAGCCCCA
GCTGCGGCGCCGGCGCTACGAGTACGACCACTGGGACGCGGCCATCCATGGCTTCAGGGAGACAGAGAAA
TCCTGCTGGTCTGATGCAAGCCAGGTCATCCTGCAGCGGGTTCGGGCGGCTGCATTTGGTCCTGACCAGA
GCCTGCTATCCCCAGTACATGTGTTGGACCTGGAACCTCGGGGCTACATCAAGCCTCATGTTGACAGTGT
CAAGTTCTGTGGATCTACCATTGCTGGCCTTTCCCTGTTGTCTCCAAGTGTTATGAAGCTGGTGCATACA
CAGGAACCTGAGCAGTGGCTGGAACTGTTGCTGGAGCCAGGGTCTCTCTATATCTTAAGGGGTTCAGCCC
GATATGACTTCTCCCATGAGATCCTTAGAGATGAAGAATCATTTTTTGGGGAGCACCGGGTTCCCCGGGG
CCGACGCATCTCAGTGATTTGCCGCTCCCTCCCTGAGGGGATGGGGCCAGGAAGGCCAGAAGAGCCACCT
CCAGCCTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR202335 protein sequence
Red=Cloning site Green=Tags(s)

MRLLSGCAWVRGSDSAVLGRLRDEAVVHPGFLSQEEEDTLTRELEPQLRRRRYEYDHWDAAIHGFRETEK
SCWSDASQVILQRVRAAAFGPDQSLLSPVHVLDLEPRGYIKPHVDSVKFCGSTIAGLSLLSPSVMKLVHT
QEPEQWLELLLEPGSLYILRGSARYDFSHEILRDEESFFGEHRVPRGRRISVICRSLPEGMGPGRPEEPP
PAC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC029677
ORF Size 639 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC029677, AAH29677
RefSeq Size 718 bp
RefSeq ORF 641 bp
Locus ID 66400
Cytogenetics 17 D
MW 24.1 kDa
Summary May function as protein hydroxylase; can catalyze auto-hydroxylation at Leu-110 (in vitro), but this activity may be due to the absence of the true substrate. Required to induce programmed necrosis in response to DNA damage caused by cytotoxic alkylating agents. Acts by triggering the collapse of mitochondrial membrane potential and loss of mitochondrial function that leads to energy depletion and cell death. ALKBH7-mediated necrosis is probably required to prevent the accumulation of cells with DNA damage. Does not display DNA demethylase activity (By similarity). Involved in fatty acid metabolism.UniProtKB/Swiss-Prot Function
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"BC029677" in other vectors (4)
SKU Description Size Price
MC206899 Alkbh7 (untagged) - Mouse alkB, alkylation repair homolog 7 (E. coli) (cDNA clone MGC:39017 IMAGE:5364485), (10ug) 10 ug
$330.00
MG202335 Alkbh7 (tGFP-tagged) - Mouse alkB, alkylation repair homolog 7 (E. coli) (cDNA clone MGC:39017 IMAGE:5364485) 10 ug
$500.00
MR202335L3 Lenti ORF clone of Alkbh7 (Myc-DDK-tagged) - Mouse alkB, alkylation repair homolog 7 (E. coli) (cDNA clone MGC:39017 IMAGE:5364485) 10 ug
$600.00
MR202335L4 Lenti ORF clone of Alkbh7 (mGFP-tagged) - Mouse alkB, alkylation repair homolog 7 (E. coli) (cDNA clone MGC:39017 IMAGE:5364485) 10 ug
$600.00

Plasmid Map for MR202335

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR202335
Circular map for MR202335

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