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Rgs4 (NM_009062) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR202147
Rgs4 (Myc-DDK-tagged) - Mouse regulator of G-protein signaling 4 (Rgs4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Rgs4 or NM_009062"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rgs4
Synonyms AA004315; AA597169; ESTM48; ESTM50
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202147 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGCAAAGGACTTGCAGGTCTGCCGGCTTCCTGCCTGAGGAGTGCAAAGGACATGAAACATCGGCTGG
GGTTCCTGCTGCAGAAGTCAGATTCCTGCGAACACAGTTCTTCACACAGCAAGAAGGACAAAGTAGTAAC
TTGCCAGAGGGTAAGCCAAGAAGAAGTCAAGAAATGGGCTGAATCGTTGGAAAACCTGATTCACCATGAA
TGTGGACTGGCAGCTTTCAAAGCTTTCCTGAAGTCGGAATACAGCGAGGAGAACATTGACTTCTGGATCA
GCTGTGAGGAGTACAAGAAGATCAAGTCACCTTCTAAACTAAGTCCCAAAGCCAAGAAGATCTACAACGA
GTTCATCTCAGTGCAAGCAACAAAAGAGGTGAACCTGGACTCTTGCACCAGAGAGGAGACAAGCCGGAAC
ATGTTACAGCCCACAATAACCTGTTTTGATGAAGCCCAGAAGAAGATTTTCAACCTGATGGAAAAGGATT
CCTACCGCCGCTTCCTCAAGTCTCGATTCTATCTTGACCTGACCAATCCTTCCAGCTGCGGGGCAGAGAA
GCAGAAAGGAGCCAAGAGTTCTGCAGACTGCACTTCCCTGGTCTCCCAGTGTGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR202147 protein sequence
Red=Cloning site Green=Tags(s)

MCKGLAGLPASCLRSAKDMKHRLGFLLQKSDSCEHSSSHSKKDKVVTCQRVSQEEVKKWAESLENLIHHE
CGLAAFKAFLKSEYSEENIDFWISCEEYKKIKSPSKLSPKAKKIYNEFISVQATKEVNLDSCTREETSRN
MLQPTITCFDEAQKKIFNLMEKDSYRRFLKSRFYLDLTNPSSCGAEKQKGAKSSADCTSLVSQCA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009062
ORF Size 615 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009062.3
RefSeq Size 2952 bp
RefSeq ORF 618 bp
Locus ID 19736
UniProt ID O08899
Cytogenetics 1 76.84 cM
MW 23.3 kDa
Summary Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein (By similarity).UniProtKB/Swiss-Prot Function
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"NM_009062" in other vectors (4)
SKU Description Size Price
MC200200 Rgs4 (untagged) - Mouse regulator of G-protein signaling 4 (Rgs4), (10ug) 10 ug
$300.00
MG202147 Rgs4 (tGFP-tagged) - Mouse regulator of G-protein signaling 4 (Rgs4) 10 ug
$489.00 $500.00
MR202147L3 Lenti ORF clone of Rgs4 (Myc-DDK-tagged) - Mouse regulator of G-protein signaling 4 (Rgs4) 10 ug
$600.00
MR202147L4 Lenti ORF clone of Rgs4 (mGFP-tagged) - Mouse regulator of G-protein signaling 4 (Rgs4) 10 ug
$600.00

Plasmid Map for MR202147

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR202147
Circular map for MR202147

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