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Cbln4 (NM_175631) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201963
Cbln4 (Myc-DDK-tagged) - Mouse cerebellin 4 precursor protein (Cbln4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
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Other products for "Cbln4 or NM_175631"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cbln4
Synonyms AI848962
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201963 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCTCCGCGCGCCGGGCGCTGTCCGTGGTACCAGCCGTTCTGCTGATTCTAGTGCTGCCTGTCTGGG
CACAGAATGACACGGAACCGATCGTTCTGGAGGGCAAGTGTCTGGTGGTGTGCGACTCGAACCCAGCTAC
AGACTCCAAGGGATCATCATCTTCCCCTCTGGGGATATCGGTCCGGGCAGCAAACTCCAAGGTGGCTTTT
TCGGCGGTTCGGAGCACCAACCACGAGCCATCTGAGATGAGCAACAAGACTCGCATCATTTACTTTGATC
AGATCCTGGTTAACGTGGGTAATTTTTTCACATTGGAATCTGTCTTTGTGGCACCGAGGAAAGGAATCTA
TAGTTTCAGTTTTCACGTAATTAAAGTCTACCAGAGCCAAACCATCCAGGTTAACTTGATGTTAAATGGA
AAACCAGTCATCTCTGCATTTGCTGGTGATAAAGATGTGACCCGTGAAGCCGCCACTAATGGAGTGCTCC
TGTACCTGGACAAAGAAGATAAGGTCTACCTAAAACTGGAGAAAGGTAACTTGCTCGGTGGCTGGCAGTA
TTCCACGTTTTCTGGCTTTCTGGTGTTTCCTCTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201963 protein sequence
Red=Cloning site Green=Tags(s)

MGSARRALSVVPAVLLILVLPVWAQNDTEPIVLEGKCLVVCDSNPATDSKGSSSSPLGISVRAANSKVAF
SAVRSTNHEPSEMSNKTRIIYFDQILVNVGNFFTLESVFVAPRKGIYSFSFHVIKVYQSQTIQVNLMLNG
KPVISAFAGDKDVTREAATNGVLLYLDKEDKVYLKLEKGNLLGGWQYSTFSGFLVFPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175631
ORF Size 594 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175631.3, NP_783439.1
RefSeq Size 2799 bp
RefSeq ORF 597 bp
Locus ID 228942
UniProt ID Q8BME9
Cytogenetics 2 H3
MW 21.6 kDa
Summary May be involved in synaptic functions in the CNS. May play a role in CBLN3 export from the endoplasmic reticulum and secretion.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175631" in other vectors (4)
SKU Description Size Price
MC207904 Cbln4 (untagged) - Mouse cerebellin 4 precursor protein (Cbln4), (10ug) 10 ug
$330.00
MG201963 Cbln4 (tGFP-tagged) - Mouse cerebellin 4 precursor protein (Cbln4) 10 ug
$500.00
MR201963L3 Lenti ORF clone of Cbln4 (Myc-DDK-tagged) - Mouse cerebellin 4 precursor protein (Cbln4) 10 ug
$600.00
MR201963L4 Lenti ORF clone of Cbln4 (mGFP-tagged) - Mouse cerebellin 4 precursor protein (Cbln4) 10 ug
$600.00

Plasmid Map for MR201963

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201963
Circular map for MR201963

file-alt Citations

Cerebellin 4, a synaptic protein that enhances inhibitory activity and resistance of neurons to Amyloid beta toxicity
Chacón, Marco, Á Arévalo
Neurobiology of Aging (2014)
AI Summary: OriGene products, specifically the pCMV6-Myc-FLAG tagged Cbln4 plasmid and the pGFP-VRS vector, were used to express and study the function of Cerebellin 4 in neuronal cells in this research.
Snippet: 2.2. Expression vectors. EGFP expressing vector (pEGFP-N1) was obtained from Biosciences/Clontech (Palo Alto, CA, USA). pCMV6-Myc-FLAG tagged Cbln4 plasmid and pGFP-VRS were obtained from Origene Technologies(Rockville, MD, USA). ... Cite SaveSaving
Showing 1-1 of 1 papers.
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