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Apopt1 (NM_026511) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201850
Apopt1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2810002N01 gene (2810002N01Rik), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Apopt1 or NM_026511"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Apopt1
Synonyms 1700081D05Rik; 2810002N01Rik; Apop-1; Apop1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201850 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTTTGCGGCCCGGAAGCAGAGCGCTTCGCCGGCTTCTCTGCCGGTCCTTCTCCGGCGGCGGCG
GCGTTAGGTTGGCCCGTGAGCGCCCTACGGATCACAGGGATGCGGCGTCCAGCCGGGTCTCAAGATTCTG
CCCTCCAAGACAGTCTTGCCATGATTGGATAGGCCCCCCAGACAAATGCTCAAACCTTCGTCCCGTTCAT
TTTCACATCCCTGAAAACGAGTCTCCCTTGGAGCAAAGACTTAGAGAATTGAGACAAGAAACGCAAGAAT
GGAATCAACAGTTCTGGGCCAAGCAGAACTTGTCCTTCAATAAGGAAAAAGAAGAATTCATTTACTCAAG
ACTACAAGCTAAGGGCGCAGGCTTGCGGACTGAGTCAGGCCAAAGAGCAACGCTGGATGCAGAGGAGATG
GCCGACTTCTATAAGGACTTTCTGAGTAAGAATTTCCAGAAGCACATGCGCTATAACCGAGACTGGTACA
AGCGTAATTTTGCCATCACTTTCTTCATGGGGAAGGTGGTCCTGGAGAGGATGTGGAGCAAGCTAAGGCA
AAAGAAGACAAGCAGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201850 protein sequence
Red=Cloning site Green=Tags(s)

MAALRPGSRALRRLLCRSFSGGGGVRLARERPTDHRDAASSRVSRFCPPRQSCHDWIGPPDKCSNLRPVH
FHIPENESPLEQRLRELRQETQEWNQQFWAKQNLSFNKEKEEFIYSRLQAKGAGLRTESGQRATLDAEEM
ADFYKDFLSKNFQKHMRYNRDWYKRNFAITFFMGKVVLERMWSKLRQKKTSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026511
ORF Size 576 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026511.2, NP_080787.1
RefSeq Size 1061 bp
RefSeq ORF 579 bp
Locus ID 68020
UniProt ID Q9CQW7
Cytogenetics 12 F1
MW 22.7 kDa
Summary Plays a role in the regulation of apoptosis. Mediates mitochondria-induced cell death in vascular smooth muscle cells through the release of cytochrome c from mitochondria, followed by the activation of the caspase cascade.UniProtKB/Swiss-Prot Function
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Documents
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"NM_026511" in other vectors (4)
SKU Description Size Price
MC207652 Apopt1 (untagged) - Mouse RIKEN cDNA 2810002N01 gene (2810002N01Rik), transcript variant 2, (10ug) 10 ug
$330.00
MG201850 Apopt1 (tGFP-tagged) - Mouse RIKEN cDNA 2810002N01 gene (2810002N01Rik) 10 ug
$500.00
MR201850L3 Lenti ORF clone of Apopt1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2810002N01 gene (2810002N01Rik), transcript variant 2 10 ug
$600.00
MR201850L4 Lenti ORF clone of Apopt1 (mGFP-tagged) - Mouse RIKEN cDNA 2810002N01 gene (2810002N01Rik), transcript variant 2 10 ug
$600.00

Plasmid Map for MR201850

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201850
Circular map for MR201850

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