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Adat2 (NM_025748) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201825
Adat2 (Myc-DDK-tagged) - Mouse adenosine deaminase, tRNA-specific 2, TAD2 homolog (S. cerevisiae) (Adat2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

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Other products for "Adat2 or NM_025748"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Adat2
Synonyms 4933426M09Rik; Deadc1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201825 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGAAGGTGGAGTCCACCACTACTCCAGACGGCCCGTGCGTGGTGTCCGTACAGGAGACGGAAA
AGTGGATGGAGGAGGCGATGCGAATGGCCAAAGAAGCTTTGGAAAATATTGAAGTTCCTGTTGGCTGCCT
TATGGTCTATAACAATGAAGTTGTGGGGAAGGGAAGAAATGAAGTGAATCAGACAAAAAACGCTACTCGG
CATGCTGAAATGGTGGCCATTGATCAGGTCCTAGACTGGTGTCATCAGCATGGACAGAGCCCTTCCACTG
TATTCGAGCACACCGTGTTGTACGTCACTGTGGAGCCGTGCATCATGTGTGCTGCCGCCCTGCGCCTTAT
GAAAATCCCGCTGGTCGTCTATGGCTGTCAGAACGAACGGTTTGGTGGTTGCGGTTCCGTCCTAAACATT
GCCTCTGCTGACCTACCCAATACTGGAAGACCGTTTCAGTGCATCCCTGGGTACCGAGCTGAGGAGGCTG
TGGAGCTCCTAAAGACCTTCTACAAACAGGAGAACCCAAATGCGCCAAAATCAAAAGTTCGGAAAAAGGA
TTGTCAGAAATCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201825 protein sequence
Red=Cloning site Green=Tags(s)

MEEKVESTTTPDGPCVVSVQETEKWMEEAMRMAKEALENIEVPVGCLMVYNNEVVGKGRNEVNQTKNATR
HAEMVAIDQVLDWCHQHGQSPSTVFEHTVLYVTVEPCIMCAAALRLMKIPLVVYGCQNERFGGCGSVLNI
ASADLPNTGRPFQCIPGYRAEEAVELLKTFYKQENPNAPKSKVRKKDCQKS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025748
ORF Size 573 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025748.4
RefSeq Size 1453 bp
RefSeq ORF 576 bp
Locus ID 66757
UniProt ID Q6P6J0
Cytogenetics 10 A2
MW 21.3 kDa
Summary Probably participates in deamination of adenosine-34 to inosine in many tRNAs.UniProtKB/Swiss-Prot Function
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Documents
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"NM_025748" in other vectors (4)
SKU Description Size Price
MC204639 Adat2 (untagged) - Mouse adenosine deaminase, tRNA-specific 2, TAD2 homolog (S. cerevisiae) (Adat2), (10ug) 10 ug
$300.00
MG201825 Adat2 (tGFP-tagged) - Mouse deaminase domain containing 1 (Deadc1) 10 ug
$500.00
MR201825L3 Lenti ORF clone of Adat2 (Myc-DDK-tagged) - Mouse adenosine deaminase, tRNA-specific 2, TAD2 homolog (S. cerevisiae) (Adat2) 10 ug
$600.00
MR201825L4 Lenti ORF clone of Adat2 (mGFP-tagged) - Mouse adenosine deaminase, tRNA-specific 2, TAD2 homolog (S. cerevisiae) (Adat2) 10 ug
$600.00

Plasmid Map for MR201825

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR201825
Circular map for MR201825

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