OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Apom (NM_018816) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR201811
Apom (Myc-DDK-tagged) - Mouse apolipoprotein M (Apom)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA305872 Goat Anti-Apolipoprotein M (mouse) Antibody
100 ug
$565.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Apom or NM_018816"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Apom
Synonyms 1190010O19Rik; G3a; NG20
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201811 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCACCAAGTTTGGGCAGCTCTGCTCTCTCTCTATGGCCTTCTCTTTAACTCCATGAATCAGTGCC
CTGAGCACAGTCAACTAACTGCGCTGGGAATGGACGACACAGAGACCCCAGAGCCCCACCTGGGCCTGTG
GTACTTTATTGCGGGAGCAGCCCCCACCACGGAAGAGTTGGCAACTTTTGATCCGGTGGACAATATTGTC
TTCAACATGGCTGCCGGCTCTGCCCCAAGGCAGCTCCAGCTTCGTGCTACCATCCGCACGAAAAGTGGGG
TCTGTGTGCCCCGGAAGTGGACATACCGATTGACTGAAGGGAAAGGAAACATGGAACTCAGAACTGAAGG
GCGCCCAGACATGAAAACAGACCTGTTCTCCAGCTCGTGCCCAGGAGGAATCATGCTGAAAGAGACGGGC
CAGGGCTACCAGCGCTTTCTCCTCTACAATCGGTCACCACACCCTCCAGAGAAGTGTGTGGAGGAATTCA
AGTCTCTGACCTCTTGCTTGGACTTCAAAGCCTTCTTAGTGACTCCCAGGAATCAAGAGGCCTGCCCGCT
GTCCAGCAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201811 protein sequence
Red=Cloning site Green=Tags(s)

MFHQVWAALLSLYGLLFNSMNQCPEHSQLTALGMDDTETPEPHLGLWYFIAGAAPTTEELATFDPVDNIV
FNMAAGSAPRQLQLRATIRTKSGVCVPRKWTYRLTEGKGNMELRTEGRPDMKTDLFSSSCPGGIMLKETG
QGYQRFLLYNRSPHPPEKCVEEFKSLTSCLDFKAFLVTPRNQEACPLSSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018816
ORF Size 570 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018816.1, NP_061286.1
RefSeq Size 731 bp
RefSeq ORF 573 bp
Locus ID 55938
UniProt ID Q9Z1R3
Cytogenetics 17 B1
MW 21.3 kDa
Summary Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_018816" in other vectors (4)
SKU Description Size Price
MC201336 Apom (untagged) - Mouse apolipoprotein M (Apom), (10ug) 10 ug
$300.00
MG201811 Apom (tGFP-tagged) - Mouse apolipoprotein M (Apom) 10 ug
$500.00
MR201811L3 Lenti ORF clone of Apom (Myc-DDK-tagged) - Mouse apolipoprotein M (Apom) 10 ug
$600.00
MR201811L4 Lenti ORF clone of Apom (mGFP-tagged) - Mouse apolipoprotein M (Apom) 10 ug
$600.00

Plasmid Map for MR201811

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201811
Circular map for MR201811

file-alt Citations

Designer high-density lipoprotein particles enhance endothelial barrier function and suppress inflammation
Lin, Swendeman, Moreira et al
Sci Signal (2024) 17 (824), eadg9256 DOI: 10.1126/scisignal.adg9256
Cited by 18
AI Summary: OriGene’s ApoM cDNA (MR201811) was used in this study to generate the ApoA1-ApoM fusion protein, enabling the design and functional testing of HDL-like nanoparticles that enhance endothelial barrier function and suppress inflammation.
Snippet: urine ApoM (MR201811) obtained from OriGene. The cDNA for ApoA1 was amplified to include the endogenous Kozak sequence and ORF of ApoA1 and to replace the stop codon with a codon for glycine. The cDNA for ApoM was amplified by sequential polymerase chain reaction (PCR) to include a linker sequence encoding one copy of a five–amino acid sequence linker (GGGGS) added to the 5′ end of ApoM
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products