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Arl6 (NM_019665) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201738
Arl6 (Myc-DDK-tagged) - Mouse ADP-ribosylation factor-like 6 (Arl6)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Arl6 or NM_019665"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Arl6
Synonyms 1110018H24Rik; 2210411E14Rik; BBS3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201738 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCTTGCTGGACAGACTTTCAGGTTTGCTTGGCCTGAAGAAGAAGGAGGTTCATGTTCTGTGCCTGG
GGCTGGATAACAGTGGGAAAACCACAATCATTAACAAGCTGAAGCCTTCCAACGCTCAATCTCAAGATAT
AGTTCCAACCATAGGATTTAGCATAGAGAAATTCAAGTCCTCCAGTTTGTCTTTTACAGTGTTTGACATG
TCAGGTCAAGGAAGGTACAGGAATCTCTGGGAACACTATTATAAAGATGGACAAGCCATTATTTTTGTCA
TTGATAGTAGTGATAAGTTAAGAATGGTTGTGGCCAAAGAAGAGCTTGATACCCTTCTGAATCACCCAGA
TATTAAGCACCGTCGAATTCCAATCTTGTTCTTTGCAAACAAAATGGATCTTAGAGACTCAGTGACCTCT
GTGAAGGTGTCTCAGTTGCTGTGCTTAGAGAGCATTAAAGACAAGCCGTGGCATATTTGTGCTAGTGACG
CCATCAAAGGAGAAGGCCTGCAGGAAGGCGTGGACTGGCTCCAAGATCAGATCCAAGCTGTGAAGACG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201738 protein sequence
Red=Cloning site Green=Tags(s)

MVLLDRLSGLLGLKKKEVHVLCLGLDNSGKTTIINKLKPSNAQSQDIVPTIGFSIEKFKSSSLSFTVFDM
SGQGRYRNLWEHYYKDGQAIIFVIDSSDKLRMVVAKEELDTLLNHPDIKHRRIPILFFANKMDLRDSVTS
VKVSQLLCLESIKDKPWHICASDAIKGEGLQEGVDWLQDQIQAVKT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019665
ORF Size 558 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019665.2, NP_062639.2
RefSeq Size 1512 bp
RefSeq ORF 561 bp
Locus ID 56297
UniProt ID O88848
Cytogenetics 16 C1.3
MW 21 kDa
Summary Involved in membrane protein trafficking at the base of the ciliary organelle (By similarity). Mediates recruitment onto plasma membrane of the BBSome complex which would constitute a coat complex required for sorting of specific membrane proteins to the primary cilia (By similarity). Together with BBS1, is necessary for correct trafficking of PKD1 to primary cilia (PubMed:24939912). Together with the BBSome complex and LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation (By similarity). May regulate cilia assembly and disassembly and subsequent ciliary signaling events such as the Wnt signaling cascade (By similarity). Isoform 2 may be required for proper retinal function and organization (PubMed:20333246).UniProtKB/Swiss-Prot Function
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"NM_019665" in other vectors (4)
SKU Description Size Price
MC201491 Arl6 (untagged) - Mouse ADP-ribosylation factor-like 6 (Arl6), (10ug) 10 ug
$300.00
MG201738 Arl6 (tGFP-tagged) - Mouse ADP-ribosylation factor-like 6 (Arl6) 10 ug
$500.00
MR201738L3 Lenti ORF clone of Arl6 (Myc-DDK-tagged) - Mouse ADP-ribosylation factor-like 6 (Arl6) 10 ug
$600.00
MR201738L4 Lenti ORF clone of Arl6 (mGFP-tagged) - Mouse ADP-ribosylation factor-like 6 (Arl6) 10 ug
$600.00

Plasmid Map for MR201738

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201738
Circular map for MR201738

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