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Ssu72 (BC016544) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201644
Ssu72 (Myc-DDK-tagged) - Mouse Ssu72 RNA polymerase II CTD phosphatase homolog (yeast) (cDNA clone MGC:27889 IMAGE:3497878)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Ssu72 or BC016544"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ssu72
Synonyms 1190002E22Rik; 1500011L16Rik; 2610101M12Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201644 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGTCATCGCCGCTGCGGGTGGCGGTGGTGTGCTCGAGTAACCAGAATCGGAGCATGGAAGCACACA
ACATCCTCAGCAAGCGAGGTTTCAGTGTCCGCTCCTTTGGAACAGGAACTCATGTGAAGCTCCCAGGACC
AGCACCTGACAAGCCCAATGTTTATGACTTCAAAACCACATATGACCAGATGTACAATGACCTCCTCAGG
AAAGACAAAGAACTCTATACACAAAATGGAATTTTACATATGTTGGACAGAAATAAAAGGATCAAGCCCC
GTCCAGAAAGGTTCCAGAACTGCACGGACCTGTTTGACTTGATCCTCACTTGTGAAGAGAGAGTCTATGA
CCAGGTTGTAGAAGATCTGAACTCCAGAGAACAGGAGACCTGCCAGCCAGTGCATGTGGTCAATGTGGAC
ATCCAGGACAACCATGAAGAGGCCACTCTGGGAGCATTCCTTATCTGCGAGCTCTGCCAGTGTGTGAGTC
TAAGCTCCTGGGTCCTGTTGGGGCTTCTCATAGCAACTTATAAAAATAAAATAAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201644 protein sequence
Red=Cloning site Green=Tags(s)

MPSSPLRVAVVCSSNQNRSMEAHNILSKRGFSVRSFGTGTHVKLPGPAPDKPNVYDFKTTYDQMYNDLLR
KDKELYTQNGILHMLDRNKRIKPRPERFQNCTDLFDLILTCEERVYDQVVEDLNSREQETCQPVHVVNVD
IQDNHEEATLGAFLICELCQCVSLSSWVLLGLLIATYKNKIK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC016544
ORF Size 546 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC016544, AAH16544
RefSeq Size 884 bp
RefSeq ORF 548 bp
Locus ID 68991
Cytogenetics 4 E2
MW 20.9 kDa
Summary Protein phosphatase that catalyzes the dephosphorylation of the C-terminal domain of RNA polymerase II. Plays a role in RNA processing and termination. Plays a role in pre-mRNA polyadenylation via its interaction with SYMPK.UniProtKB/Swiss-Prot Function
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"BC016544" in other vectors (4)
SKU Description Size Price
MC206927 Ssu72 (untagged) - Mouse Ssu72 RNA polymerase II CTD phosphatase homolog (yeast) (cDNA clone MGC:27889 IMAGE:3497878), (10ug) 10 ug
$330.00
MG201644 Ssu72 (tGFP-tagged) - Mouse Ssu72 RNA polymerase II CTD phosphatase homolog (yeast) (cDNA clone MGC:27889 IMAGE:3497878) 10 ug
$500.00
MR201644L3 Lenti ORF clone of Ssu72 (Myc-DDK-tagged) - Mouse Ssu72 RNA polymerase II CTD phosphatase homolog (yeast) (cDNA clone MGC:27889 IMAGE:3497878) 10 ug
$600.00
MR201644L4 Lenti ORF clone of Ssu72 (mGFP-tagged) - Mouse Ssu72 RNA polymerase II CTD phosphatase homolog (yeast) (cDNA clone MGC:27889 IMAGE:3497878) 10 ug
$600.00

Plasmid Map for MR201644

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201644
Circular map for MR201644

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