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Il17b (NM_019508) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201619
Il17b (Myc-DDK-tagged) - Mouse interleukin 17B (Il17b)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Il17b
Synonyms 1110006O16Rik; 1700006N07Rik; Zcyto7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201619 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTGGCCGCACAGCCTGCTCTTCCTCCTGGCCATCTCCATCTTCCTGGCGCCAAGCCACCCCCGGA
ACACCAAAGGCAAAAGAAAAGGGCAAGGGAGGCCCAGTCCCTTGGCCCCTGGGCCTCATCAGGTGCCGCT
GGACCTGGTGTCTCGAGTAAAGCCCTACGCTCGAATGGAAGAGTATGAGCGGAACCTTGGGGAGATGGTG
GCCCAGCTGAGGAACAGCTCCGAGCCAGCCAAGAAGAAATGTGAAGTCAATCTACAGCTGTGGTTGTCCA
ACAAGAGGAGCCTGTCCCCATGGGGCTACAGCATCAACCACGACCCCAGCCGCATCCCTGCGGACTTGCC
CGAGGCGCGGTGCCTATGTTTGGGTTGCGTGAATCCCTTCACCATGCAGGAGGACCGTAGCATGGTGAGC
GTGCCAGTGTTCAGCCAGGTGCCGGTGCGCCGCCGCCTCTGTCCTCAACCTCCTCGCCCTGGGCCCTGCC
GCCAGCGTGTCGTCATGGAGACCATCGCTGTGGGTTGCACCTGCATCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201619 protein sequence
Red=Cloning site Green=Tags(s)

MDWPHSLLFLLAISIFLAPSHPRNTKGKRKGQGRPSPLAPGPHQVPLDLVSRVKPYARMEEYERNLGEMV
AQLRNSSEPAKKKCEVNLQLWLSNKRSLSPWGYSINHDPSRIPADLPEARCLCLGCVNPFTMQEDRSMVS
VPVFSQVPVRRRLCPQPPRPGPCRQRVVMETIAVGCTCIF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019508
ORF Size 540 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019508.1, NP_062381.1
RefSeq Size 692 bp
RefSeq ORF 543 bp
Locus ID 56069
UniProt ID Q9QXT6
Cytogenetics 18 E1
MW 20.3 kDa
Summary Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1.UniProtKB/Swiss-Prot Function
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Documents
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"NM_019508" in other vectors (4)
SKU Description Size Price
MC200167 Il17b (untagged) - Mouse interleukin 17B (Il17b), (10ug) 10 ug
$300.00
MG201619 Il17b (tGFP-tagged) - Mouse interleukin 17B (Il17b) 10 ug
$500.00
MR201619L3 Lenti ORF clone of Il17b (Myc-DDK-tagged) - Mouse interleukin 17B (Il17b) 10 ug
$600.00
MR201619L4 Lenti ORF clone of Il17b (mGFP-tagged) - Mouse interleukin 17B (Il17b) 10 ug
$600.00

Plasmid Map for MR201619

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201619
Circular map for MR201619

file-alt Citations

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