Nip7 (NM_025391) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR201608
Nip7 (Myc-DDK-tagged) - Mouse nuclear import 7 homolog (S. cerevisiae) (Nip7), transcript variant 1
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nip7
Synonyms 1110017C15Rik; 6330509M23Rik; AA408773; AA410017; PEachy
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201608 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCCCTTGACTGAAGAAGAGACCCGGGTCATGTTTGAGAAGATCGCGAAGTACATCGGAGAGAACC
TGCAACTCCTAGTGGACAGACCCGACGGCACGTACTGTTTCCGGCTGCACAACGACCGAGTGTACTATGT
GAGTGAGATGATGCTGAAGCTGGCCGCCAACATCTCCGGAGACAAGCTCGTGTCGCTGGGGACGTGTTTT
GGGAAGTTTACCAAGACCCACAAGTTCCGCTTGCACGTGACAGCTCTGGATTACCTGGCCCCCTATGCCA
AGTATAAAGTGTGGGTAAAGCCGGGAGCAGAGCAGTCCTTCTTGTATGGAAACCATGTGTTGAAATCCGG
TCTGGGTCGAATTACTGAGAACACATCTCAGTACCAGGGAGTGGTTGTCTATTCCATGGCGGACATTCCT
CTGGGTTTTGGGGTGGCAGCAAAGTCTACCCAAGACTGCCGAAAAGTGGACCCCATGGCAATTGTGGTGT
TCCACCAAGCAGACATTGGGGAGTACGTACGCCATGAAGAGACACTGACT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR201608 protein sequence
Red=Cloning site Green=Tags(s)

MRPLTEEETRVMFEKIAKYIGENLQLLVDRPDGTYCFRLHNDRVYYVSEMMLKLAANISGDKLVSLGTCF
GKFTKTHKFRLHVTALDYLAPYAKYKVWVKPGAEQSFLYGNHVLKSGLGRITENTSQYQGVVVYSMADIP
LGFGVAAKSTQDCRKVDPMAIVVFHQADIGEYVRHEETLT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025391
ORF Size 540 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025391.1, NM_025391.2, NP_079667.1, NP_079667.2
RefSeq Size 3036 bp
RefSeq ORF 543 bp
Locus ID 66164
UniProt ID Q9CXK8
Cytogenetics 8 D3
MW 20.5 kDa
Summary Required for proper 34S pre-rRNA processing and 60S ribosome subunit assembly.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025391" in other vectors (4)
SKU Description Size Price
MC207567 Nip7 (untagged) - Mouse nuclear import 7 homolog (S. cerevisiae) (Nip7), transcript variant 1, (10ug) 10 ug
$330.00
MG201608 Nip7 (tGFP-tagged) - Mouse nuclear import 7 homolog (S. cerevisiae) (Nip7) 10 ug
$500.00
MR201608L3 Lenti ORF clone of Nip7 (Myc-DDK-tagged) - Mouse nuclear import 7 homolog (S. cerevisiae) (Nip7), transcript variant 1 10 ug
$600.00
MR201608L4 Lenti ORF clone of Nip7 (mGFP-tagged) - Mouse nuclear import 7 homolog (S. cerevisiae) (Nip7), transcript variant 1 10 ug
$600.00

Plasmid Map for MR201608

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Cloning scheme for MR201608

Circular map for MR201608

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