Tcf21 (NM_011545) Mouse Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Tcf21 |
| Synonyms | bHLHa23; capsulin; epc; epicardin; Pod-1; Pod1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_011545 |
| ORF Size | 537 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_011545.2 |
| RefSeq Size | 1240 bp |
| RefSeq ORF | 540 bp |
| Locus ID | 21412 |
| UniProt ID | O35437 |
| Cytogenetics | 10 10.33 cM |
| MW | 20.1 kDa |
| Summary | Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis. May be involved in the organogenesis of the spleen and heart and in cardiac and coronary artery development. May function in the development and sex differentiation of gonad via transcriptional regulation of AD4BP/SF-1.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_011545" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC202295 | Tcf21 (untagged) - Mouse transcription factor 21 (Tcf21), (10ug) | 10 ug |
$450.00
|
|
| MG201596 | Tcf21 (tGFP-tagged) - Mouse transcription factor 21 (Tcf21) | 10 ug |
$650.00
|
|
| MR201596L3 | Lenti ORF clone of Tcf21 (Myc-DDK-tagged) - Mouse transcription factor 21 (Tcf21) | 10 ug |
$750.00
|
|
| MR201596L4 | Lenti ORF clone of Tcf21 (mGFP-tagged) - Mouse transcription factor 21 (Tcf21) | 10 ug |
$750.00
|
|
Citations
Tcf21 modulates fibroblast activation and promotes cardiac fibrosis after injury via Pdgfrb signaling
Baba, Maezawa, Kondo et al
Sci Rep (2025) 15 (1), 28260 DOI: 10.1038/s41598-025-13102-3
Baba, Maezawa, Kondo et al
Sci Rep (2025) 15 (1), 28260 DOI: 10.1038/s41598-025-13102-3
Cited by 7
Snippet: 419 bp from TSS were used. These loci were identified from the TCF21 binding sites by ChIP-seq and prediction of H3K4me3 and H3K27ac peaks from CHIP-Atlas. We transferred the Tcf21 Mouse ORF Clone (OriGene, MR201596) to the pTRE3G vector and electroporated it into pEFa-Tet3G-stable expressing 3T3-L1 cells (Tet-On 3G Inducible Expression System, Clontech). We selected hygromycin-resistant cells a
Showing 1-1 of 1 papers.
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