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Praf2 (NM_138602) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201577
Praf2 (Myc-DDK-tagged) - Mouse PRA1 domain family 2 (Praf2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Praf2 or NM_138602"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Praf2
Synonyms AA986329; C78013; DXImx39e; JM4; Sfc20
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201577 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGAGGTGCGGCTGCCACCGCTGCGCGCCCTAGACGACTTTGTCCTGGGGTCTGCGCGCCTGGCGG
CTCCTGATCCAGGCGATCCACAACGATGGTGTCACCGCGTCATCAACAACCTCCTCTACTACCAAACCAA
TTACCTTCTCTGCTTTGGCATCAGTCTTGCGCTGGCCGGGTACATTCGTCCGCTGCACACCCTCCTGAGT
GCACTGGTAGTGGTGGTGGCCCTCGGGGTGCTGGTGTGGGCTGCTGAAACCCGTGCAGCTGTGCGCCGCT
GCCGTCGCAGCCACCCTGCTGCCTGCCTGGCTGCAGTGCTTGCCATTAGCCTCTTCATTCTCTGGGCCGT
GGGTGGCGCCTTCACCTTCCTGCTCAGCATCACAGCGCCTGTGTTTTTGATCCTGCTGCATGCTTCACTG
CGTCTGAGAAACCTTAAAAACAAGATTGAGAACAAGATCGAGAGCATCGGTCTAAAGCGGACACCAATGG
GATTGCTTCTAGAGGCACTGGGACAGGAGCAGGAAGCTGGATCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201577 protein sequence
Red=Cloning site Green=Tags(s)

MSEVRLPPLRALDDFVLGSARLAAPDPGDPQRWCHRVINNLLYYQTNYLLCFGISLALAGYIRPLHTLLS
ALVVVVALGVLVWAAETRAAVRRCRRSHPAACLAAVLAISLFILWAVGGAFTFLLSITAPVFLILLHASL
RLRNLKNKIENKIESIGLKRTPMGLLLEALGQEQEAGS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138602
ORF Size 534 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138602.4
RefSeq Size 1206 bp
RefSeq ORF 537 bp
Locus ID 54637
UniProt ID Q9JIG8
Cytogenetics X 3.48 cM
MW 19.5 kDa
Summary May be involved in ER/Golgi transport and vesicular traffic. Plays a proapoptotic role in cerulenin-induced neuroblastoma apoptosis (By similarity).UniProtKB/Swiss-Prot Function
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"NM_138602" in other vectors (4)
SKU Description Size Price
MC201916 Praf2 (untagged) - Mouse PRA1 domain family 2 (Praf2), (10ug) 10 ug
$300.00
MG201577 Praf2 (tGFP-tagged) - Mouse PRA1 domain family 2 (Praf2) 10 ug
$489.00 $500.00
MR201577L3 Lenti ORF clone of Praf2 (Myc-DDK-tagged) - Mouse PRA1 domain family 2 (Praf2) 10 ug
$600.00
MR201577L4 Lenti ORF clone of Praf2 (mGFP-tagged) - Mouse PRA1 domain family 2 (Praf2) 10 ug
$600.00

Plasmid Map for MR201577

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR201577
Circular map for MR201577

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