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Ppih (NM_028677) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201558
Ppih (Myc-DDK-tagged) - Mouse peptidyl prolyl isomerase H (Ppih), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Ppih or NM_028677"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ppih
Synonyms 1100001J08Rik; 2010111B15Rik; 4833408F11Rik; AI464484; CYP-20; CYPH; D4Wsu43e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201558 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGTGGCAAATTCAAGTCCAGTCAATCCAGTGGTCTTCTTCGATGTCAGCATTGGCGGCCAGGAAG
TTGGTCGCATGAAAATCGAGCTCTTTGCAGACGTGGTGCCTAAGACGGCAGAGAACTTTAGGCAATTCTG
CACCGGAGAGTTCAGAAAAGATGGCGTTCCGATAGGATACAAAGGAAGCACCTTCCACAGGGTCATAAAG
GATTTCATGATTCAGGGTGGAGATTTTGTTAATGGCGATGGCACTGGAGTCGCCAGTATTTACCGGGGTC
CATTTGCGGATGAAAATTTTAAACTTAGACACTCTGCTCCAGGCCTGCTTTCCATGGCAAACAGTGGTCC
CAGTACAAATGGCTGCCAGTTCTTTATCACGTGTTCTAAGTGTGATTGGCTGGATGGAAAGCATGTAGTG
TTTGGAAAAATCATTGACGGACTTCTAGTGATGAGGAAGATCGAGAATGTTCCCACAGGCCCCAACAATA
AGCCCAAACTGCCAGTGGTGATCTCACAGTGTGGGGAAATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201558 protein sequence
Red=Cloning site Green=Tags(s)

MAVANSSPVNPVVFFDVSIGGQEVGRMKIELFADVVPKTAENFRQFCTGEFRKDGVPIGYKGSTFHRVIK
DFMIQGGDFVNGDGTGVASIYRGPFADENFKLRHSAPGLLSMANSGPSTNGCQFFITCSKCDWLDGKHVV
FGKIIDGLLVMRKIENVPTGPNNKPKLPVVISQCGEM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_028677
ORF Size 531 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028677.4, NP_082953.1
RefSeq Size 795 bp
RefSeq ORF 534 bp
Locus ID 66101
UniProt ID Q9D868
Cytogenetics 4 55.34 cM
MW 19.2 kDa
Summary PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. May act as a chaperone.UniProtKB/Swiss-Prot Function
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"NM_028677" in other vectors (4)
SKU Description Size Price
MC207560 Ppih (untagged) - Mouse peptidyl prolyl isomerase H (Ppih), transcript variant 1, (10ug) 10 ug
$330.00
MG201558 Ppih (tGFP-tagged) - Mouse peptidyl prolyl isomerase H (Ppih) 10 ug
$500.00
MR201558L3 Lenti ORF clone of Ppih (Myc-DDK-tagged) - Mouse peptidyl prolyl isomerase H (Ppih), transcript variant 1 10 ug
$600.00
MR201558L4 Lenti ORF clone of Ppih (mGFP-tagged) - Mouse peptidyl prolyl isomerase H (Ppih), transcript variant 1 10 ug
$600.00

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