Ndufb10 (NM_026684) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR201544
Ndufb10 (Myc-DDK-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10 (Ndufb10), nuclear gene encoding mitochondrial protein
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ndufb10
Synonyms 22kDa; 0610011B04Rik; PDSW
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201544 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAGATTCTTGGGACAAGGATGTGTACCCGGAGCCCCCGAGCCGCACGCCTGCTCCCTCGCCGCAGA
CCTCGCTCCCTAACCCCATCACCTACTTGACGAAGGCCTACGACCTCGTCGTGGACTGGCCCGTGACCCT
CGTGAGAGAGTTTATTGAACGACAGCATGCCAAGAACCGAACCTACTACTACCACCGACAGTACCGTCGA
GTGCCAGACATCACAGAATGCAAAGAGGGTGATGTTCTGTGTATCTATGAGGCTGAGATGCAGTGGAGAA
GGGACTTCAAAGTTGACCAGGAGATCATGAACATCATCCAGGAGAGACTGAAGGCTTGTCAGCAGAGGGA
GGGAGAGAACTACCAGCAGAACTGTGCCAAGGAACTGGAGCAGTTCACCAAAGTGACTAAAGCCTACCAG
GACCGCTACTTGGACCTGGGAGCCTATTATTCTGCCAGAAAGTGTCTGGCAAAGCAGAAGCAGAGAATGC
TGGAAGAAAGGAAGGCTGCGAGGCAGGAGGCTGCTGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR201544 protein sequence
Red=Cloning site Green=Tags(s)

MPDSWDKDVYPEPPSRTPAPSPQTSLPNPITYLTKAYDLVVDWPVTLVREFIERQHAKNRTYYYHRQYRR
VPDITECKEGDVLCIYEAEMQWRRDFKVDQEIMNIIQERLKACQQREGENYQQNCAKELEQFTKVTKAYQ
DRYLDLGAYYSARKCLAKQKQRMLEERKAARQEAAA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026684
ORF Size 528 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026684.2
RefSeq Size 657 bp
RefSeq ORF 531 bp
Locus ID 68342
UniProt ID Q9DCS9
Cytogenetics 17 A3.3
MW 21 kDa
Summary Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026684" in other vectors (4)
SKU Description Size Price
MC202225 Ndufb10 (untagged) - Mouse NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10 (Ndufb10), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$300.00
MG201544 Ndufb10 (tGFP-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10 (Ndufb10) 10 ug
$500.00
MR201544L3 Lenti ORF clone of Ndufb10 (Myc-DDK-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10 (Ndufb10), nuclear gene encoding mitochondrial protein 10 ug
$600.00
MR201544L4 Lenti ORF clone of Ndufb10 (mGFP-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10 (Ndufb10), nuclear gene encoding mitochondrial protein 10 ug
$600.00

Plasmid Map for MR201544

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Cloning scheme for MR201544

Circular map for MR201544

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