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Resp18 (NM_009049) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201527
Resp18 (Myc-DDK-tagged) - Mouse regulated endocrine-specific protein 18 (Resp18)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

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Other products for "Resp18 or NM_009049"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Resp18
Synonyms AI851012
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201527 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGAGTTCGTTAAAGCCCGCGGGCTCCGGGCATCTTCAGCTACTGGTCTGCTTCCTTCTGCTTTACA
GTCGTCCAGGCAGCTGCAGCGACATAAACGCCCACGATGGTCAGGGCCAAGTGGGATCGGAACAGCTCTG
GACATTCCAAGGACTTATTGCTTCAGTCTTCCAGTACTTACAACTTATATTCCACCAGATTGTACCCGAA
GGCATGTTCTGGGCAGATGACATAGCCTATGAGTTAATGACCAAAAAGGTGGAGCACCTCAGCAGACTCC
ACCCCCAGTATCCATGCAGGAAGGATATGAAGGCAGTTTCCCCCACGGCAAACGCTGGGGTGAGGAGCAA
GCAAGAGGAGAAACTTCAACTCTTATCTCCCCAAAAGAGTCCAACAGTCAAGGTGAACAGGGACCGATGC
TTTACCACCAAAGTAATCCCAAAGGCCACCAAACAAGAGGCCACCCATCCTACCAAGGGTTTCTTTGGGC
CATTCCCCACTGTGGGCCTCAACCTTGTTGCTGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201527 protein sequence
Red=Cloning site Green=Tags(s)

MQSSLKPAGSGHLQLLVCFLLLYSRPGSCSDINAHDGQGQVGSEQLWTFQGLIASVFQYLQLIFHQIVPE
GMFWADDIAYELMTKKVEHLSRLHPQYPCRKDMKAVSPTANAGVRSKQEEKLQLLSPQKSPTVKVNRDRC
FTTKVIPKATKQEATHPTKGFFGPFPTVGLNLVAD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009049
ORF Size 525 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009049.2
RefSeq Size 710 bp
RefSeq ORF 528 bp
Locus ID 19711
UniProt ID P47939
Cytogenetics 1 C4
MW 19.5 kDa
Summary This gene encodes a secreted protein that is expressed mainly in the peripheral endocrine and neuroendocrine tissues and is regulated by physiological factors that include blood glucose and dopaminergic drugs. The encoded protein is found in the lumen of the endoplasmic reticulum and is degraded in the post-ER pre-Golgi compartment. Gene knockout experiments in mice demonstrate that this gene is essential for embryonic development with embryonic lethality occurring before embryonic day 9.5. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2016
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"NM_009049" in other vectors (4)
SKU Description Size Price
MC207377 Resp18 (untagged) - Mouse regulated endocrine-specific protein 18 (Resp18), (10ug) 10 ug
$330.00
MG201527 Resp18 (tGFP-tagged) - Mouse regulated endocrine-specific protein 18 (Resp18) 10 ug
$500.00
MR201527L3 Lenti ORF clone of Resp18 (Myc-DDK-tagged) - Mouse regulated endocrine-specific protein 18 (Resp18) 10 ug
$600.00
MR201527L4 Lenti ORF clone of Resp18 (mGFP-tagged) - Mouse regulated endocrine-specific protein 18 (Resp18) 10 ug
$600.00

Plasmid Map for MR201527

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201527
Circular map for MR201527

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