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Myl9 (NM_172118) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201455
Myl9 (Myc-DDK-tagged) - Mouse myosin, light polypeptide 9, regulatory (Myl9)
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Myl9 or NM_172118"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Myl9
Synonyms AI327049; MLC20; Mylc2c; RLC-C
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201455 representing NM_172118
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGAGCAAGAGAGCCAAGGCCAAGACCACCAAGAAGAGGCCCCAGAGGGCTACGTCCAATGTCTTCG
CCATGTTTGACCAGTCCCAGATCCAGGAGTTTAAGGAGGCCTTCAACATGATTGATCAGAACCGAGATGG
CTTCATTGATAAGGAGGACCTGCACGACATGCTGGCCTCTCTGGGGAAGAACCCCACAGACGAGTATCTG
GAGGGCATGATGAACGAGGCGCCAGGGCCCATCAACTTCACCATGTTCCTCACAATGTTTGGGGAGAAGC
TGAACGGCACAGACCCCGAGGATGTGATCCGCAATGCCTTTGCCTGCTTTGATGAGGAGGCCTCAGGCTT
CATCCACGAGGACCACCTGAGGGAGCTGCTCACCACCATGGGCGACCGATTCACGGATGAGGAGGTGGAC
GAGATGTACCGCGAGGCACCCATTGATAAGAAGGGCAACTTCAACTACGTGGAGTTCACTCGCATCCTCA
AACACGGCGCCAAGGACAAGGACGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201455 representing NM_172118
Red=Cloning site Green=Tags(s)

MSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYL
EGMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVD
EMYREAPIDKKGNFNYVEFTRILKHGAKDKDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172118
ORF Size 516 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172118.1, NP_742116.1
RefSeq Size 1083 bp
RefSeq ORF 519 bp
Locus ID 98932
UniProt ID Q9CQ19
Cytogenetics 2 H1
MW 20.3 kDa
Summary Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.UniProtKB/Swiss-Prot Function
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"NM_172118" in other vectors (3)
SKU Description Size Price
MC212039 Myl9 (untagged) - Mouse myosin, light polypeptide 9, regulatory (Myl9), (10ug) 10 ug
$330.00
MR201455L3 Lenti ORF clone of Myl9 (Myc-DDK-tagged) - Mouse myosin, light polypeptide 9, regulatory (Myl9) 10 ug
$600.00
MR201455L4 Lenti ORF clone of Myl9 (mGFP-tagged) - Mouse myosin, light polypeptide 9, regulatory (Myl9) 10 ug
$600.00

Plasmid Map for MR201455

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201455
Circular map for MR201455

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