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Polr2g (NM_026329) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201451
Polr2g (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide G (Polr2g)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Polr2g or NM_026329"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Polr2g
Synonyms 2410046K11Rik; A230108L04Rik; C76415; RBP7; Rpo2-7l
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201451 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTTATCACATTTCCCTGGAGCACGAGATCCTCCTGCACCCACGATACTTCGGTCCAAACTTGCTCA
ACACGGTGAAGCAGAAGCTGTTTACCGAGGTGGAGGGGACCTGCACTGGGAAATATGGCTTTGTAATTGC
TGTCACCACCATCGACAATATTGGTGCTGGTGTGATCCAGCCAGGCCGAGGTTTTGTTCTTTATCCAGTG
AAATACAAAGCTATTGTTTTCCGGCCCTTTAAAGGTGAAGTGGTGGATGCTGTGGTCACTCAGGTCAACA
AGGTTGGACTTTTCACAGAAATTGGGCCTATGTCTTGCTTCATCTCTCGACATTCCATCCCTTCAGAGAT
GGAGTTTGATCCAAATTCAAACCCCCCTTGCTATAAGACCATGGACGAGGACATTGTGATTCAGCAGGAC
GATGAGATACGCTTGAAGATTGTAGGCACGCGTGTAGACAAGAATGACATTTTTGCCATTGGCTCTCTGA
TGGACGACTACTTGGGGCTGGTGAGC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201451 protein sequence
Red=Cloning site Green=Tags(s)

MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPV
KYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFDPNSNPPCYKTMDEDIVIQQD
DEIRLKIVGTRVDKNDIFAIGSLMDDYLGLVS

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_026329
ORF Size 516 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026329.1, NM_026329.2, NP_080605.1
RefSeq Size 868 bp
RefSeq ORF 519 bp
Locus ID 67710
UniProt ID P62488
Cytogenetics 19 A
MW 19.3 kDa
Summary DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. Binds RNA (By similarity).UniProtKB/Swiss-Prot Function
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"NM_026329" in other vectors (4)
SKU Description Size Price
MC200405 Polr2g (untagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide G (Polr2g), (10ug) 10 ug
$300.00
MG201451 Polr2g (tGFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide G (Polr2g) 10 ug
$500.00
MR201451L3 Lenti ORF clone of Polr2g (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide G (Polr2g) 10 ug
$600.00
MR201451L4 Lenti ORF clone of Polr2g (mGFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide G (Polr2g) 10 ug
$600.00

Plasmid Map for MR201451

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201451
Circular map for MR201451

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