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Sdhc (NM_025321) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201415
Sdhc (Myc-DDK-tagged) - Mouse succinate dehydrogenase complex, subunit C, integral membrane protein (Sdhc), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sdhc
Synonyms 0610010E03Rik; AI316496; AU019277
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201415 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCGCTCTTGCTGAGACATGTCGGCCGTCACTGCCTCCGAGCCCACCTGAATGCTCAGCTTTGTA
TCAGAAATGCTGCTCCTTTGGGAACCACAGCTAAGGAGGAGATGGAGCGGTTCTGGAAGAAGAACACGAG
TTCAAACCGTCCTCTGTCTCCCCATTTGACTATCTACAAATGGTCTCTTCCTATGGCACTGTCCGTTTGC
CACCGAGGCTCTGGAATAGCCTTGAGTGGAGGGGTCTCTCTTTTTGGCCTGTCGGCACTGGTGCTTCCTG
GGAACTTTGAGTCGTATTTGATGTTTGTGAAGTCCCTGTGTTTGGGGCCAACACTGATCTACTCGGCTAA
GTTTGTGCTTGTCTTCCCGCTCATGTACCACTCACTGAATGGGATCCGACACTTGCTATGGGACCTAGGA
AAAGGCCTGGCAATACCCCAGGTCTGGCTGTCTGGAGTGGCGGTCGTGGTTCTTGCTGTGTTGTCCTCTG
GCGGGCTGGCCGCCCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201415 protein sequence
Red=Cloning site Green=Tags(s)

MAALLLRHVGRHCLRAHLNAQLCIRNAAPLGTTAKEEMERFWKKNTSSNRPLSPHLTIYKWSLPMALSVC
HRGSGIALSGGVSLFGLSALVLPGNFESYLMFVKSLCLGPTLIYSAKFVLVFPLMYHSLNGIRHLLWDLG
KGLAIPQVWLSGVAVVVLAVLSSGGLAAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025321
ORF Size 507 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025321.1, NM_025321.2, NM_025321.3, NP_079597.1, NP_079597.2
RefSeq Size 1179 bp
RefSeq ORF 510 bp
Locus ID 66052
UniProt ID Q9CZB0
Cytogenetics 1 H3
MW 18.3 kDa
Summary Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025321" in other vectors (4)
SKU Description Size Price
MC207555 Sdhc (untagged) - Mouse succinate dehydrogenase complex, subunit C, integral membrane protein (Sdhc), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$330.00
MG201415 Sdhc (tGFP-tagged) - Mouse succinate dehydrogenase complex, subunit C, integral membrane protein (Sdhc) 10 ug
$500.00
MR201415L3 Lenti ORF clone of Sdhc (Myc-DDK-tagged) - Mouse succinate dehydrogenase complex, subunit C, integral membrane protein (Sdhc), nuclear gene encoding mitochondrial protein 10 ug
$600.00
MR201415L4 Lenti ORF clone of Sdhc (mGFP-tagged) - Mouse succinate dehydrogenase complex, subunit C, integral membrane protein (Sdhc), nuclear gene encoding mitochondrial protein 10 ug
$600.00

Plasmid Map for MR201415

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201415
Circular map for MR201415

file-alt Citations

Synthetic Lethal Screening for Genetic Vulnerabilities in a Succinate Dehydrogenase-Loss Model of Paraganglioma
Al Khazal
Thesis (2026)
AI Summary: OriGene plasmids MR203816 (Sdhb) and MR201415 (Sdhc) were used as templates to PCR amplify SDH subunit genes for cloning in the study.
Snippet: Sdhb and Sdhc were PCR amplified from OriGene plasmids MR203816 and MR201415, respectively. Gibson (NEB) cloning was used to insert Sdhb or Sdhc into the pKTol2C-EGFP plasmid (14). Both SDH subunit
Approaches to reduce succinate accumulation by restoration of succinate dehydrogenase activity in cultured adrenal cells
Khazal, Rahimi, Feng et al
J Cell Sci (2025)
Cited by 1
AI Summary: OriGene plasmids MR203816 (Sdhb) and MR201415 (Sdhc) were used as templates to amplify and clone mouse Sdhb and Sdhc genes for gene replacement experiments, enabling the study of succinate dehydrogenase restoration in adrenal cell models.
Snippet: f the appropriate transposase. Mouse Sdhb and Sdhc were PCR amplified from OriGene plasmids MR203816 and MR201415, respectively. Gibson (NEB) cloning was used to insert Sdhb or Sdhc into the pKTol2C-EGFP plasmid (Clark et al., 2007). Both SDH subunit plasmids contain a MYC-DDK epitope tag on their 3’ termini to allow for Western blot mo
Showing 1-2 of 2 papers.
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