Avp (NM_009732) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR201383
Avp (Myc-DDK-tagged) - Mouse arginine vasopressin (Avp)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Avp
Synonyms Vp; Vsp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201383 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCGCCAGGATGCTCAACACTACGCTCTCCGCTTGTTTCCTGAGCCTGCTGGCCTTCTCCTCCGCCT
GCTACTTCCAGAACTGCCCAAGAGGCGGCAAGAGGGCCATCTCTGACATGGAGCTGAGACAGTGTCTCCC
CTGCGGCCCGGGCGGCAAAGGACGCTGCTTCGGACCAAGCATCTGCTGCGCGGACGAGCTGGGCTGCTTC
GTGGGCACCGCCGAGGCGCTGCGCTGCCAGGAGGAGAACTACCTGCCCTCGCCCTGCCAGTCCGGCCAGA
AGCCCTGCGGGAGCGGGGGCCGCTGCGCCGCCGTGGGCATCTGCTGCAGCGACGAGAGCTGCGTGGCCGA
GCCCGAGTGCCACGACGGTTTTTTCCGCCTCACCCGCGCTCGGGAGCCAAGCAACGCCACACAGCTGGAC
GGCCCTGCTCGGGCGCTGCTGCTAAGGCTGGTACAGCTGGCTGGGACACGGGAGTCCGTGGATTCTGCCA
AGCCCCGGGTCTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR201383 protein sequence
Red=Cloning site Green=Tags(s)

MLARMLNTTLSACFLSLLAFSSACYFQNCPRGGKRAISDMELRQCLPCGPGGKGRCFGPSICCADELGCF
VGTAEALRCQEENYLPSPCQSGQKPCGSGGRCAAVGICCSDESCVAEPECHDGFFRLTRAREPSNATQLD
GPARALLLRLVQLAGTRESVDSAKPRVY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009732
ORF Size 504 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009732.2
RefSeq Size 507 bp
RefSeq ORF 507 bp
Locus ID 11998
UniProt ID P35455
Cytogenetics 2 63.24 cM
MW 17.9 kDa
Summary This gene encodes a member of the vasopressin/oxytocin family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the neuropeptide hormone arginine vasopressin, and two other peptides, neurophysin 2 and copeptin. Arginine vasopressin binds to vasopressin receptors and functions as a vasopressor, to constrict blood vessels and increase blood pressure, and as an antidiuretic, to reduce the production of urine. Neurophysin 2 functions as a carrier protein in the transport of arginine vasopressin. This gene is present in a gene cluster with the related gene oxytocin on chromosome 2. provided by RefSeq, Aug 2015
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Documents
Resources
"NM_009732" in other vectors (4)
SKU Description Size Price
MC202013 Avp (untagged) - Mouse arginine vasopressin (Avp), (10ug) 10 ug
$300.00
MG201383 Avp (tGFP-tagged) - Mouse arginine vasopressin (Avp) 10 ug
$500.00
MR201383L3 Lenti ORF clone of Avp (Myc-DDK-tagged) - Mouse arginine vasopressin (Avp) 10 ug
$600.00
MR201383L4 Lenti ORF clone of Avp (mGFP-tagged) - Mouse arginine vasopressin (Avp) 10 ug
$600.00

Plasmid Map for MR201383

Download sequence for

Cloning scheme for MR201383

Circular map for MR201383

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